Fig 2: Representative immunohistochemical examination patterns of the expression of CHRM3 and ACh-E of non-lesional skin before provocation in HC (A–D) and CholU patients (E–H). (A, D) DAPI staining of nuclei of sweat glands; (B, E) CHRM3 staining (red); (C, F) ACh-E staining (FITC) d/g CHRM3 and ACh-E overlay.

Fig 3: Representative immunohistochemical examination patterns of the expression of CHRM3 of HC and CholU patients separated per sweating group: (a/ c/ e/ g) non-lesional skin samples taken before provocation, (b) non-lesional skin after provocation from a sample of the HC group, and (d/ f/ h) lesional skin samples after provocation from CholU patients. Zoom-in boxes depict the cutouts in higher magnifications. HC, healthy controls; NS, normal sweating CholU; RS, reduced sweating CholU; SRS, severely reduced sweating CholU.