Anti-DYKDDDDK Tag (L5) Affinity Gel 651502 from BioLegend

Product Specs
ItemAnti-DYKDDDDK Tag (L5) Affinity Gel
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number651502
CloneL5
ApplicationsPurification, Immunoprecipitation (IP)
Quantity2 mL
Conjugate/TagUnconjugated
HostRat
Format50% anti-DYKDDDDK-tag (L5) antibody conjugated resin is supplied in 1X PBS and 0.09% NaN₃. The volume specified for each catalog number indicates the volume of resin included
ImmunogenDYKDDDDK-tagged mouse Langerin
ClonalityMonoclonal
ReactivityEpitope tag
Antibody TypePrimary
TargetDYKDDDDK
IsotypeIgG2a, Lambda

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BioLegend

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San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Plant-produced RBD and cocktail-based vaccine candidates are highly effective against SARS-CoV-2, independently of its emerging variantsFront Plant SciAugust 2, 2023Tarlan Mamedov, Damla Yuksel, Irem Gurbuzaslan, Merve Ilgin, Burcu Gulec, Gulshan Mammadova, Aykut Ozdarendeli, Shaikh Terkis Islam Pavel, Hazel Yetiskin, Busra Kaplan, et al.Mamedov et al., 2021b). Plant-produced antigens were purified using anti-DYKDDDDK affinity gel (cat. no. 651503, BioLegend). gRBD-, dRBD-, and N+RBD-expressing leaves weighing 20 g were extracted in 1× PBS buffer

(2) The mitophagy receptor NIX induces vIRF-1 oligomerization and interaction with GABARAPL1 for the promotion of HHV-8 reactivation-induced mitophagyPLoS pathogensJuly 1, 2023Vo MT, Choi CY, Choi YB.Flag-immunoprecipitation, whole cell extracts were incubated with affinity gel (anti-DYKDDDDK tag (L5) gel, BioLegend, 651502) at 4°C overnight. Immunoprecipitants were washed with RIPA buffer, and bound proteins were eluted by

(3) Kindlin2 enables EphB/ephrinB bi-directional signaling to support vascular developmentLife Science AllianceMarch 1, 2023Wenqing Li, Lai Wen, Bhavisha Rathod, Anne-Claude Gingras, Klaus Ley, Ho-Sup Leewere developed in the laboratory. Rabbit anti-HA (923501) and anti-DYKDDDDK Tag (L5) Affinity Gel (651502) antibodies were purchased from BioLegend. Secondary Alexa Fluor–labeled antibodies were from Jackson

(4) Signal sequences encode information for protein folding in the endoplasmic reticulumThe Journal of cell biologyJanuary 2, 2023Sun S, Li X, Mariappan M.were purchased as follows: Strep-Tactin XT beads (#2-4010-010; IBA), Rat anti-FLAG L5 affinity gel (#651503; Biolegend), Protein A agarose (#CA-PRI-0100; Repligen), Mouse anti-HA magnetic beads (#88837; Pierce),

(5) Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapyCell reportsNovember 1, 2022Wang W, Wu S, Cen Z, Zhang Y, Chen Y, Huang Y, Cillo AR, Prokopec JS, Quarato G, Vignali DAA, et al.(cells lyased by RIPA buffer) were subjected to standard flag-IP, using anti-flag beads (BioLegend, 651502). Beads after IP were eluted by SDS loading buffer and then analyzed by Western blot by anti-flag (BioLegend,

(6) Trimeric receptor-binding domain of SARS-CoV-2 acts as a potent inhibitor of ACE2 receptor-mediated viral entryiScienceAugust 19, 2022Shrikanth C. Basavarajappa, Angela Rose Liu, Anna Bruchez, Zhenlu Li, Vinicius G. Suzart, Zhonghua Liu, Yinghua Chen, Tsan Sam Xiao, Matthias Buck, Parameswaran RamakrishnanPurified anti-DYKDDDDK tag antibody,BioLegend,637319 Anti-DYKDDDDK tag (L5) affinity gel antibody FLAG beads,BioLegend,651501 Anti-FLAG M2 affinity Gel,Millipore Sigma,#A2220 Monoclonal Anti-HIS tag antibody produced in mouse,Sigma Aldrich

(7) Identification of a BRAF/PA28γ/MEK1 signaling axis and its role in epithelial-mesenchymal transition in oral submucous fibrosisCell death & diseaseAugust 12, 2022Xie C, Li Z, Hua Y, Sun S, Zhong L, Chen Q, Feng H, Ji N, Li T, Zhou X, et al.as an input control. The lysates were incubated with 20 μL of anti-Flag tag affinity gel antibody (651502, Biolegend, CA, USA) overnight at 4 °C. The precipitated protein complex was harvested by centrifugation,

(8) Snapshots of actin and tubulin folding inside the TRiC chaperoninNature Structural & Molecular BiologyMay 1, 2022John J. Kelly, Dale Tranter, Els Pardon, Gamma Chi, Holger Kramer, Lotta Happonen, Kelly M. Knee, Jay M. Janz, Jan Steyaert, Christine Bulawa, et al.1 hour with 5 ml of anti-FLAG resin (Anti-DYKDDDK Tag (L5) Affinity Gel Protocol BioLegend (cat no. 651503). Resin/lysate mixture was passed through a gravity column, washed with 100 CV of wash buffer (20 mM

(9) Astrocytic laminin-211 drives disseminated breast tumor cell dormancy in brainNature CancerJanuary 1, 2022Jinxiang Dai et al.supernatant per sample was immunoprecipitated with anti-DYKDDDDK FLAG Tag (L5) Affinity Gel (Biolegend, 651501) or control rat IgG agarose (Rockland, 012–0050), at 4°C overnight. The beads were washed 3 times in

(10) Production and Characterization of Nucleocapsid and RBD Cocktail Antigens of SARS-CoV-2 in Nicotiana benthamiana Plant as a Vaccine Candidate against COVID-19Vaccines (Basel)November 17, 2021Tarlan Mamedov et al.CA, USA), and recombinant proteins N and N+RBD were detected with anti-DYKDDDDK antibody (cat. no. 651503, BioLegend) or human novel coronavirus nucleoprotein (N) (1–419 aa) monoclonal antibody (MBS7135930,

(11) Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastasesNature communicationsOctober 21, 2021Raman V, Van Dessel N, Hall CL, Wetherby VE, Whitney SA, Kolewe EL, Bloom SMK, Sharma A, Hardy JA, Bollen M, et al.homogenized, and incubated overnight with 50 µl of anti-FLAG purification resin (Biolegend, catalog # 651502). Beads were boiled for 5 min and loaded onto SDS-PAGE gels. β-actin was identified with mouse anti-actin

(12) Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in MiceVirusesAugust 12, 2021Tarlan Mamedov et al.proteins were performed by anti-FLAG affinity chromatography using anti-DYKDDDDK affinity gel (cat. no. 651503, BioLegend) as described previously [37]. For purification, 20 g of frozen leaves, infiltrated with

(13) A Molecular Mechanism for Turning Off IRE1α Signaling during Endoplasmic Reticulum StressCell ReportsDecember 29, 2020Xia Li, Sha Sun, Suhila Appathurai, Arunkumar Sundaram, Rachel Plumb, Malaiyalam MariappanHegde,N/A Resins,, mouse anti-HA magnetic beads,Fisher Scientific,88836 Rat anti-FLAG beads,Biolegend,651503 StrepTactin-XT Sepharose,"IBA, Germany",2-1201-010 Protein A agarose,Repligen,CA-PRI-1000 Reagents,, DMEM,Corning,10–013-CV FBS,GIBCO,16000044 Horse Serum

(14) Mechanosensing through YAP controls T cell activation and metabolismThe Journal of experimental medicineAugust 3, 2020Meng KP, Majedi FS, Thauland TJ, Butte MJ.buffer and 10× reducing agent. For FLAG pulldown, anti-DYKDDDDK affinity resin (clone L5, BioLegend, 651501) was blocked for an hour with PBS + 1% BSA and incubated with 500 µg lysate in 300 µl overnight at 4°C.

(15) Phosphoglycerate Kinase 1 Phosphorylates Beclin1 to Induce AutophagyMolecular cellMarch 2, 2017Qian X, Li X, Cai Q, Zhang C, Yu Q, Jiang Y, Lee JH, Hawke D, Wang Y, Xia Y, et al.

(16) Splicing factor SF3B1K700E mutant dysregulates erythroid differentiation via aberrant alternative splicing of transcription factor TAL1PloS oneJanuary 1, 2017Jin S, Su H, Tran NT, Song J, Lu SS, Li Y, Huang S, Abdel-Wahab O, Liu Y, Zhao X.sc-9741) antibodies were purchased from Santa Cruz biotech. Anti-Flag affinity gel is from Biolegend (651501). AntiRBM15 (10587-1-AP) antibody is from Proteintech. SF3B1 antibody (PAS-19679) is from Fisher Scientific.

(17) In vivo production of non-glycosylated recombinant proteins in Nicotiana benthamiana plants by co-expression with Endo-β-N-acetylglucosaminidase H (Endo H) of Streptomyces plicatusPloS oneJanuary 1, 2017Mamedov T, Cicek K, Gulec B, Ungor R, Hasanova G.Endo H was performed by anti-FLAG affinity chromatography using anti-DYKDDDDK affinity gel (Cat. No. 651503, BioLegend). For purification, 7 g of frozen leaves was ground in 20 mL PBS buffer (1XPBS, 150 mM NaCI)

(18) Regulation of the ryanodine receptor by anti-apoptotic Bcl-2 is independent of its BH3-domain-binding propertiesBiochemical and biophysical research communicationsJuly 31, 2015Vervliet T, Lemmens I, Welkenhuyzen K, Tavernier J, Parys JB, Bultynck G.

(19) 14-3-3ε and ζ regulate neurogenesis and differentiation of neuronal progenitor cells in the developing brainThe Journal of neuroscience : the official journal of the Society for NeuroscienceSeptember 3, 2014Toyo-oka K, Wachi T, Hunt RF, Baraban SC, Taya S, Ramshaw H, Kaibuchi K, Schwarz QP, Lopez AF, Wynshaw-Boris A.

(20) Transport of misfolded endoplasmic reticulum proteins to the cell surface by MHC class II moleculesInternational immunologyApril 1, 2013Jiang Y, Arase N, Kohyama M, Hirayasu K, Suenaga T, Jin H, Matsumoto M, Shida K, Lanier LL, Saito T, et al.

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Anti-DYKDDDDK Tag (L5) Affinity Gel from BioLegend

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Anti-DYKDDDDK Tag (L5) Affinity Gel from BioLegend

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