Western blot analysis was performed on Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), NTERA-2 (Lane 2), NIH/3T3 (Lane 3), Hep G2 (Lane 4), K-562 (Lane 5), SH-SY5Y (Lane 6), HEK-293 (Lane 7) and A-431 (Lane 8). The blots were probed with Anti-SNRPB Monoclonal Antibody (Product # MA5-13446, 1 µg/mL) and detected by chemiluminescence. A 24 kDa and 30 kDa band corresponding to Smith antigen was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).