Fig 2: Effect of inhibitors rDDK-1 and LY374973 on Wnt and Notch pathway molecules in transplantation tumor model detected by western blot assay. (A) Effects of rDDK-1 and LY374973 on Wnt pathway molecules β-catenin, Pra-1 and c-myc. (B) Effects of rDDK-1 and LY374973 on Notch pathway molecules Notch1, Notch2, Jagged and DLL4.

Fig 3: PTH‐induced Notch1 pathway activation by regulating GSAP. (A) The site prediction of miR‐29a‐5p with GSAP. (B) The dual‐luciferase assay miR‐29a‐5p with GSAP. n = 10 per group. *** P < .01 vs mimics‐NC group. (C, D) The protein level of GSAP in groups of HUVECs and CKD rats respectively. (E) The level of γ‐secretase in the CTL, PTH, PTH+PTHrP(7‐34), PTH+miR‐29a‐mimics and PTH+si‐GSAP groups. (F, G) The protein level of NICD and Hes1 and in the CTL, PTH, PTH+miR‐29a‐5p‐mimics, PTH+miR‐NC, PTH+NS and PTH+si‐GSAP groups. (H, I) Fluorescence staining of Hes1. (J) The pattern diagram of Notch1 signal activation regulated by miR‐29a‐5p/GSAP. (C‐H) Data were presented as the mean ± SD. n = 4 per group. ** P < .01 vs CTL group. *** P < .001 vs CTL group. #P < .01 vs PTH group. ##P < .01 vs PTH group. ###P < .001 vs PTH group

Fig 4: Role of the Notch signaling pathway in DM-MI in vivo. Total protein was extracted from myocardial tissues and Notch1, Notch4, DLL1, Jagged1, MAML1 and p300 protein expression levels were determined by western blotting. β-actin served as a loading control. Data are expressed as the mean ± standard deviation. **P<0.01. STZ, streptozotocin; I/R, ischemia/reperfusion; DM, diabetes mellitus; MI, myocardial infarction; DLL1, Delta-like 1; MAML1, Mastermind-like protein 1; ns, not significant.

Fig 5: Notch1 is a direct target of miR-708 in gastric cancer. (A) The putative wild-type (Wt) and mutant (Mut) miR-708 binding sequences in the 3′-untranslated region (3′-UTR) of Notch1 are shown. (B, C) BGC-823 and SGC-7901 cells were cotransfected with miR-708 mimics or miR-NC and luciferase reporter plasmid carrying Wt or Mut binding sites for miR-708 in the 3′-UTR of Notch1. Then 48 h posttransfection, cells were harvested and subjected to the analysis of luciferase activity using dual-luciferase reporter assays. *p < 0.05 compared with miR-NC. (D, E) RT-qPCR and Western blot analysis were conducted to measure the expression of Notch1 at the mRNA and protein levels, respectively, in BGC-823 and SGC-7901 cells transfected with miR-708 mimics or miR-NC. *p < 0.05 compared with miR-NC.