Fig 1: Phenotypic characterization of expanded Tregs. Gates were set on CD4+CD25+ cells. Cell surface expression of CD25, CD127, CD62L, GITR and HLA-DR, and intracellular expression of Foxp3 and CTLA-4 in Xeno-Treg or Poly-Treg are presented as the percentage of CD4+CD25+ cells co-expressing individual Treg markers examined. Data presented in figures represent the percentage of CD4+CD25+ cells co-expressing individual Treg markers tested. Data are representative of three independent experiments. CD, cluster of differentiation; CTLA-4, cytotoxic T-lymphocyte antigen-4; FoxP3, forkhead box P3; GITR, glucocorticoid-induced tumor necrosis factor receptor-related protein; HLA-DR, human leukocyte antigen-DR; Poly-Treg, polyclonal Treg; Tregs, regulatory T cells; Xeno-Treg, Treg with xenoantigen specificity.