Fig 1: Over-expression of TTP impairs pancreatic cancer cell growth in vivo and in vitroPancreatic cancer cell lines (PanC-1 and AsPC-1) were transfected with a TTP over-expression vector and transfection efficiency was confirmed by western blot A. TTP over-expression vector-transfected PanC-1 and AsPC-1 cell viability was reduced compared to controls B. TTP over-expression reduced colony formation compared to controls C. Results from three independent experiments are shown. TTP over-expression reduced tumor weights compared to controls D. **P < 0.05, *P < 0.01 VS control.
Fig 2: TTP over-expression inhibits proliferation and increases apoptosis in pancreatic cancer cellsApoptosis is increased A. and BrdU positivity is decreased B. in PanC-1 cells over-expressing TTP compared to the control.
Fig 3: RNA-seq analysis of candidate genes in TTP over-expressing PanC-1 and AsPC-1 cellsExpression patterns of RNAs in TTP control/TTP over-expressing PanC-1 and AsPC-1 cells were shown in the left with a heat map (unsupervised clustering). And a selected group of RNAs were also showed in the right with a zoomed view. The data are colored red or green for high abundance/upregulation or abundance/downregulation.
Fig 4: Low TTP expression predicts poor patient outcomeKaplan-Meier curves for overall survival (OS) of 90 pancreatic cancer patients A. (B) Kaplan-Meier curves for OS in 90 pancreatic cancer patients with high or low TTP expression B.
Fig 5: TTP over-expression downregulates Pim-1 and IL-6 in pancreatic cancer cell linesPim-1 and IL-6 mRNA expression is downregulated in TTP over-expressing PanC-1 and AsPC-1 cells as compared to control cells A. and B. Pim-1 and IL-6 mRNA was increased in 35 pancreatic cancer tissues as compared to paired normal tissues as shown by qRT-PCR C. Pim-1 and IL-6 protein levels were increased in tumor tissues as compared to normal tissues in four randomly chosen matched tissue pairs, as measured by western blot (T = tumor tissue; N = normal tissue) D.
Supplier Page from Abcam for Anti-Tristetraprolin/TTP antibody [OTI1A2]