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Antibodies
Anti-3-Nitrotyrosine antibody [7A12AF6]
Anti-3-Nitrotyrosine antibody [7A12AF6] from Abcam
Be the first to write a review!
Citations:
(24)
Supplier Page
Supplier Page from
Abcam for
Anti-3-Nitrotyrosine antibody [7A12AF6]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-3-Nitrotyrosine antibody [7A12AF6]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab110282
Isotype
IgG2b
Clone
7A12AF6
Clonality
Monoclonal
Quantity
100 UG
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In-Cell ELISA, Western blot
Conjugate/Tag
Unconjugated
Host
Mouse, Nude mouse
Antibody Type
Primary
Format
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation
Concentration
1 mg/ml
Reactivity
Species independent
Target
3-Nitrotyrosine
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (24)
(1)
The NO Answer for Autism Spectrum Disorder
Advanced Science
May 22, 2023
Manish Kumar Tripathi, Shashank Kumar Ojha, Maryam Kartawy, Wajeha Hamoudi, Ashwani Choudhary, Shani Stern, Adi Aran, Haitham Amal
cocktail (#
5872
) were purchased from
Cell Signaling Technology
(Danvers, MA, USA). Anti‐3Ntyr antibody (
ab110282
), Anti‐homer 1 (
ab184955
), Anti‐
NMDAR1
antibody (
ab109182
),
Anti‐SLC32A1/VGAT
antibody (1b235952), and
(2)
Human sperm proteome reveals the effect of environmental borne seminal polyaromatic hydrocarbons exposome in etiology of idiopathic male factor infertility
Frontiers in Cell and Developmental Biology
May 16, 2023
Jasmine Nayak, Soumya Ranjan Jena, Sugandh Kumar, Sujata Kar, Anshuman Dixit, Luna Samanta
HSP90β (sc-59578, Santacruz,
Mouse
), anti-4-HNE (
ab46545
, Rabbit,
Abcam
) and anti-3-nitro-tyrosine (
ab110282
,
Mouse
,
Abcam
) For maintaining biological and technical variability, triplicate tests were performed
(3)
Apoptotic vesicles resist oxidative damage in noise-induced hearing loss through activation of FOXO3a-SOD2 pathway
Stem cell research & therapy
April 15, 2023
Huang X, Kou X, Zhan T, Wei G, He F, Mao X, Yang H.
incubated with anti-4-hydroxynonenal (4-HNE,
Abcam
, #
ab46545
,
1:200
), anti-3-nitrotyrosine (3-NT,
Abcam
, #
ab110282
,
1:400
) overnight at 4 °C and secondary antibody (
CST
, #
8889
,
1:400
) for 1 h at room temperature. Then
(4)
AIF Overexpression Aggravates Oxidative Stress in Neonatal Male Mice After Hypoxia–Ischemia Injury
Molecular Neurobiology
November 1, 2022
Tao Li et al.
donkey
serum in PBS for 30 min, the monoclonal
mouse
anti-3-NT (1.5:1000 dilution, 7A12AF6,
Abcam
,
ab110282
), polyclonal rabbit anti-DCX (
1:500
dilution,
Abcam
,
ab18723
), or monoclonal mouse anti-BrdU (
1:200
(5)
Mitochondrial-Targeted Therapies Require Mitophagy to Prevent Oxidative Stress Induced by SOD2 Inactivation in Hypertrophied Cardiomyocytes
Antioxidants (Basel, Switzerland)
April 6, 2022
Peugnet V, Chwastyniak M, Mulder P, Lancel S, Bultot L, Fourny N, Renguet E, Bugger H, Beseme O, Loyens A,
et al
.
software (Zen 2.3) (Zeiss). 2.7. Antibodies The primary antibodies used were 3-nitrotyrosine (
mouse
,
ab110282
,
Abcam
, Cambridge, UK, 1/1000 WB); aconitase 2 (rabbit, GTX109736, CliniSciences, Nanterre, Paris, France,
(6)
Treatment with the Ferroptosis Inhibitor Ferrostatin-1 Attenuates Noise-Induced Hearing Loss by Suppressing Ferroptosis and Apoptosis
Oxidative medicine and cellular longevity
January 1, 2022
Ma PW, Wang WL, Chen JW, Yuan H, Lu PH, Gao W, Ding XR, Lun YQ, Liang R, He ZH,
et al
.
antibodies at 4°C overnight: 4-HNE antibody (
GeneTex
,
GTX17571
;
1 : 100
) and 3-NT antibody (
Abcam
,
ab110282
;
1 : 100
). After 3 washes with PBS, the specimens were stained with Alexa Fluor 594-conjugated donkey
(7)
Decreased proteasomal cleavage at nitrotyrosine sites in proteins and peptides
Redox Biology
October 1, 2021
Christiane Ott, Florencia Tomasina, Nicolás Campolo, Silvina Bartesaghi, Mauricio Mastrogiovanni, Alejandro Leyva, Carlos Batthyány, Walter Meinl, Tilman Grune, Rafael Radi
following antibodies were used: anti-Cyt c antibody (#136F3, Cell Signaling) and anti-NO2Y antibody (
ab110282
,
Abcam
). Fluorescent-conjugated secondary antibodies (Li-COR Biosciences) were used according to the
(8)
Deficiency of Klc2 Induces Low-Frequency Sensorineural Hearing Loss in C57BL/6 J Mice and Human
Molecular neurobiology
September 1, 2021
Fu X, An Y, Wang H, Li P, Lin J, Yuan J, Yue R, Jin Y, Gao J, Chai R.
mab1622
), anti-caspase3 (
CST
,
9661S
), anti-
Lc3b
(
CST
,
2775S
), anti-prestin (sc-22692), anti-3NT (
Abcam
,
ab110282
), anti-4HNE (
Abcam
,
ab46545
), anti-
Kcnma1
(
Alomone
,
APC-021
), anti-HAtag (
CST
,
3724S
). AAV preparation
(9)
Central Acting Hsp10 Regulates Mitochondrial Function, Fatty Acid Metabolism, and Insulin Sensitivity in the Hypothalamus
Antioxidants (Basel)
April 30, 2021
Kristina Wardelmann et al.
Total OXPHOS Rodent WB Antibody Cocktail
(
Abcam
#
ab110413
,
1:2000
), GFAP (Abcam, Cambridge, UK #7260), 3-Nitrotyrosine (
Abcam
#
ab110282
,
1:1000
), β Actin+HRP (
Sigma-Aldrich
A3854
,
1:10,000
).
Membranes
were incubated with peroxidase-conjugated
(10)
Ebselen reduces cigarette smoke‐induced endothelial dysfunction in mice
British Journal of Pharmacology
April 1, 2021
Kurt Brassington, Stanley M. H. Chan, Huei Jiunn Seow, Aleksandar Dobric, Steven Bozinovski, Stavros Selemidis, Ross Vlahos
nitroprusside (
Thermo Fisher
, USA);
eNOS
/NOS3 RRID: AB_2533121 (Thermo Fisher Scientific, USA), 3‐NT RRID:
AB110282
(
Abcam
, USA);
Goat anti‐mouse IgG (H + L) secondary antibody
, Alexa Flour Plus 488 RRID: AB_2633275
(11)
Effect of TTLL12 on tubulin tyrosine nitration as a novel target for screening anticancer drugs
in vitro
Oncology Letters
October 8, 2020
Lingli Xue, Yan Zeng, Chuan Fang, Wei Cheng, Yadong Li
Abcam
), tributyl phosphate (
TBP
) (
1:800
; cat. no.
ab220788
;
Abcam
) and nitrotyrosine (
1:1,000
; cat. no.
ab110282
;
Abcam
) at a temperature of 37°C for 3 h. Following the primary
incubation
,
membranes
were incubated
(12)
TGF-β1 attenuates mitochondrial bioenergetics in pulmonary arterial endothelial cells via the disruption of carnitine homeostasis
Redox biology
September 1, 2020
Sun X, Lu Q, Yegambaram M, Kumar S, Qu N, Srivastava A, Wang T, Fineman JR, Black SM.
anti-nitrotyrosine antibody was obtained from EMD Millipore (Billerica, MA). The anti-3-Nitrotyrosine antibody (cat#:
ab110282
) was obtained from
Abcam
(Cambridge, MA). The anti-CrAT was obtained from Proteintech (Rosemont, IL).
(13)
Age-Related Maintenance of the Autophagy-Lysosomal System Is Dependent on Skeletal Muscle Type
Oxidative Medicine and Cellular Longevity
July 24, 2020
Raquel Fernando, José Pedro Castro, Tanina Flore, Stefanie Deubel, Tilman Grune, Christiane Ott
K63-linkage specific polyubiquitin (
Cell Signaling
no.
5621
), rabbit polyclonal 3-nitrotyrosine (
Abcam
,
ab110282
),
mouse
monoclonal pentosidine (
BioLogo
,
PEN012
) and rabbit monoclonal
LAMP1
(
Cell Signaling
no.
3243
).
(14)
Genetically modified macrophages accomplish targeted gene delivery to the inflamed brain in transgenic Parkin Q311X(A) mice: importance of administration routes
Scientific reports
July 16, 2020
Haney MJ, Zhao Y, Fay J, Duhyeong H, Wang M, Wang H, Li Z, Lee YZ, Karuppan MK, El-Hage N,
et al
.
separation.
Immunoblots
were labeled with primary antibodies against 3-
Nitrotyrosine
(NT)—
1:200
(
Abcam
,
ab110282
), PSD-95—
1:200
(
Abcam
,
ab76115
), NMDAR2B—
1:200
(
Abcam
,
ab65783
), NF-kB—
1:200
(
Santa Cruz Biotechnology
,
(15)
Aging affects sex- and organ-specific trace element profiles in mice
Aging (Albany NY)
July 3, 2020
Kristina Lossow et al.
blocking solution with 0.1 %
Tween 20
(
Merck
) and incubated overnight at 4°C.
Mouse
anti-3-NT (
Abcam
,
ab110282
,
1:1,000
), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH;
Abcam
,
ab37168
,
1:10,000
),
mouse
(16)
Exogenous PP2A inhibitor exacerbates the progression of nonalcoholic fatty liver disease via NOX2-dependent activation of miR21
American Journal of Physiology-Gastrointestinal and Liver Physiology
October 1, 2019
Muayad Albadrani et al.
The primary antibody for
CD68
(
1:300
,
ab125212
,
Abcam
), ␣-SMA (
1:250
,
ab32575
,
Abcam
), 3NT (
1:250
,
ab110282
,
Abcam
), P-65 (
1:300
,
SC-8008
,
Santa Cruz Biotechnology
), and p-AKT (
1:300
,
SC-293125
,
Santa Cruz Biotechnology
)
(17)
A Delphinidin-Enriched Maqui Berry Extract Improves Bone Metabolism and Protects against Bone Loss in Osteopenic Mouse Models
Antioxidants (Basel, Switzerland)
September 10, 2019
Nagaoka M, Maeda T, Chatani M, Handa K, Yamakawa T, Kiyohara S, Negishi-Koga T, Kato Y, Takami M, Niida S,
et al
.
anti-I-κB antibody (E130,
ab32518
,
Abcam
, Cambridge, UK), and
mouse
anti-nitrotyrosine antibody (7A12AF6,
ab110282
; recognize only protein-bound nitrotyrosine,
Abcam
) followed by appropriate Alexa Fluor 488® or 594®-labeled
(18)
Low-protein diet supplemented with ketoacids delays the progression of diabetic nephropathy by inhibiting oxidative stress in the KKAy mice model
British Journal of Nutrition
January 14, 2018
Dongmei Liu, Ming Wu, Lin Li, Xiang Gao, Bo Yang, Shuqin Mei, Lili Fu, Changlin Mei
polyvinylidene difluoride
membrane
, and then incubated with the primary antibody against nitrotyrosin (NT) (
ab110282
;
Abcam
)(40). The enhancedchemiluminescence system was used to detect the signals. The concentration
(19)
Age-related oxidative changes in pancreatic islets are predominantly located in the vascular system
Redox Biology
December 29, 2017
Richard Kehm, Jeannette König, Kerstin Nowotny, Tobias Jung, Stephanie Deubel, Sabrina Gohlke, Tim Julius Schulz, Annika Höhn
anti-iNOS antibody (
ab178945
,
Abcam
, Cambridge, United Kingdom) and mouse anti-3-Nitrotyrosine antibody (
ab110282
,
Abcam
, Cambridge, United Kingdom) were used as primary antibodies for
IF staining
of PDX-1, p16Ink4a,
(20)
Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis
PloS one
January 1, 2015
Moghimi E, Solomon JA, Gianforcaro A, Hamadeh MJ.
3-NY, GPx1,
Bax
,
Bcl-2
, pro-caspase 3) against 4-HNE (
1:800
;
Abcam
,
ab46545
), 3-NY (
1:1000
;
Abcam
,
ab110282
), SOD2 (
1:8000
;
Abcam
,
ab13533
), catalase PLOS ONE | DOI:10.1371/journal.pone.0126355 May 28, 2015 4
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Anti-3-Nitrotyrosine antibody [7A12AF6] from Abcam
Supplier Page
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Product Specs
Company Info
Citations
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