Fig 1: Differentiated and matured hALNs show markers of human ARC neurons. (A) Gene expression of early and late hypothalamic progenitor markers, and nonhypothalamic markers (n = 2–3, data are shown as means ± SEM; * p < 0.05), and (B) gene expression of neuropeptide markers and in hNSC, hDHN, hMHALN and hALN (n = 2–3, data are shown as means ± SEM; * p < 0.05). (C) Immunostainings of hALNs for (from left to right and top to bottom) MAP2, synapsin (SYN), Vgat and HUC, NPY, AgRP, NPFFR2, POMC and HUC, CRH and TH (scale bar 20 µm).