Fig 1: Tumor-suppressing functions of TPM1 in OSCC cells.The plasmid (M02-TPM1), which encoded TPM1, and empty plasmid were transfected into cells. (a) Western blotting and RT-PCR were applied to evaluate the protein and mRNA levels of TPM1 8 hours after the cells were transfected, separately. (b) The inhibitory effect of TPM1 on cell proliferation in SCC15 cells and SCC25 cells was evaluated by an MTS assay; the data were collected at 0 h, 24 h, 48 h, and 72 h (Data are expressed as the mean±standard deviation. Data were obtained from 6 distinct assays; *p<0.05; **p<0.01). (c) The promoting effect of TPM1 on cell apoptosis in SCC15 cells and SCC25 cells was evaluated by an Annexin V assay, and both early stage apoptosis and total stage apoptosis were promoted (Data are expressed as the mean±standard deviation. Data were obtained from triplicate assays; *p <0.05; **p<0.01). (d) The inhibitory effect of TPM1 on cell invasion in SCC15 cells and SCC25 cells was evaluated by a Transwell assay (Data are expressed as the mean±standard deviation. Data were obtained from triplicate assays; *p<0.05; **p<0.01).
Fig 2: TPM1 expression pattern in OSCC.(a) The TPM1 mRNA level in OSCC and OSCC adjacent normal tissues from 7 patients was measured using a real-time PCR assay (Data are expressed as the mean±standard deviation. Data were obtained from triplicate assays; *p<0.05; **p<0.01). (b) Western blotting and RT-PCR were applied for evaluating the protein and mRNA levels of TPM1 in OSCC cell lines and a keratinocyte cell line (Data are expressed as the mean±standard deviation. Data were obtained from triplicate assays; *p<0.05; **p<0.01). (c) Representative images of TPM1 protein levels detected by immunohistochemical staining in OSCC tissue samples (N: OSCC adjacent normal tissue) and in OSCC tissues of patients who had follow-up information. Kaplan-Meier analysis estimated oral cancer-specific survival and overall survival, according to the TPM1 levels in OSCC patients.
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