Fig 1: miR-128-3p is down-regulated in BCSCs and breast cancer cells. A, Relative expression of miR-128-3p in breast cancer tissues and adjacent tissues (*P < 0.05 compared with adjacent tissue); B, Relative expression of miR-128-3p in breast cell lines (*P < 0.05 compared with MCF-10A); C, Relative expression of miR-128-3p in non-spheroids and spheroids (*P < 0.05 compared with non-spheroids); D, Relative expression of miR-128-3p in CD44-CD24- and CD44+CD24- (*P < 0.05 compared with CD44-CD24-); U6 was used as the internal reference of the expression level of miR-128-3p and GAPDH was as the internal reference of NEK2, Wnt, ß-catenin, CD24, CD44, Nanog, Oct-4 and Pygo2. Themeasurement data are expressed in the form of mean ± standard deviation. Difference analysis between two groups was performed by t test, and the data analysis among multiple groups was performed by one-way analysis of variance