GAP43 Antibody PA1-16729 from Thermo Fisher Scientific

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Cell death & disease
October 15 2022

Fig 1: CASZ1b is essential for neuronal differentiation.A Western blot shows induction of CASZ1b expression in CASZ1b stably transfected SY5Y cells after tetracycline (Tet) treatment for 24 h (left panel); CASZ1b represses SY5Y cell proliferation is observed based on IncuCyte cell confluence assay. B Western blot shows induction of CASZ1b expression in CASZ1b stably transfected AS cells after Tet treatment for 24 h (left panel); CASZ1b represses AS cell proliferation is observed based on IncuCyte cell confluence assay (right panel). C Realtime PCR results show that the restoration of CASZ1b upregulates mRNA levels of neuronal genes in both SY5Y cells (48 h Tet treatment) and AS cells (72 h Tet treatment). D CASZ1b induces neurite extension (yellow arrow) in SY5Y cells shown by GAP43 staining. E Western blot shows that CASZ1 is upregulated when SY5Y cells are treated with retinoic acid for 3 days. The densitometry graph on the right is generated by normalizing GAPDH signal and the signal in Ctrl is set as 1. F Western blot shows that the knockdown of CASZ1 decreases expression of both CASZ1a and CASZ1b isoforms. G The knockdown of CASZ1 attenuates RA-induced neurite extension (red arrow) shown by the phase-contrast imaging. H The silencing of CASZ1 results in a significant decrease in RA-induced neurite length as evaluated by using the IncuCyte neurite-length assay. I Realtime PCR results show that the siRNA knockdown of CASZ1 for 2 days attenuates RA induced increases in GAP43 mRNA levels. Data represent mean ± SEM, n = 3 biological replicates. Two-sided Student’s t test was used to calculate statistical difference.
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Cell reports
May 10 2022

Fig 2: Adam12 and Gap43 expression identify immune-responsive muscle-resident mesenchymal cell populations(A and B) Single-cell (A) feature and (B) violin plots for cluster 5 (Adam12) and cluster 6 (Gap43) genes and type 2 immune-receptor genes (Il4ra, Il13ra1).(C) LipidTOX/DAPI-stained PDGFRa+ sorted (C1) ADAM12+ cluster 5, (C2) GAP43+ cluster 6, (C3) CLU+ cluster 7, (C4) HSD11B1 clusters 8/9 cells cultured in adipogenic medium for 5 days.(D) LipidTOX/DAPI-stained PDGFRa+ sorted (D1) ADAM12+ cluster 5, (D2) GAP43+ cluster 6, (D3) CLU+ cluster 7, (D4) HSD11B1 clusters 8/9 cells cultured in adipogenic medium supplemented with recombinant IL-4 for 5 days.(E) LipidTOX/DAPI-stained PDGFRa+ sorted (E1) ADAM12+ cluster 5, (E2) GAP43+ cluster 6, (E3) CLU+ cluster 7, (E4) HSD11B1 clusters 8/9 cells cultured in adipogenic medium supplemented with recombinant IL-13 for 5 days.(F) Quantification of adipogenic differentiation as visualized by %LipidTOX/DAPI. Data are represented as mean ± SEM. Intergroup analysis performed with two-way ANOVA and post hoc Tukey’s test. Intragroup analysis performed with paired Student’s t test. n = 9 per cluster and condition. Significance denoted by an asterisk.(G) Flow-cytometry contour plots for PDGFRa and either IL-4Ra (cluster 5/6), CLU (cluster 7), or HSD11B1 (cluster 8/9) gated on tdT+ live cells obtained from BaCl2-injured muscle and NaCl-injected contralateral controls at 4 days post injury as well as glycerol-injured muscle and NaCl-injected contralateral controls 3 days post injury.(H) Percent quantification of tdT+/PDGFRa+/IL4Ra (cluster 5/6), tdT+/PDGFRa+/CLU+ (cluster 7), and tdT+/PDGFRa+/HSD11B1 (cluster 8/9) cells in BaCl2-injured muscle or NaCl-injected contralateral controls. n = 6 per cluster and condition. Data are represented as mean ± SEM. Significance determined using two-way ANOVA and post hoc Tukey’s test. Significance denoted by an asterisk. NS, not significant.(I) Percent quantification of tdT+/PDGFRa+/IL4-Ra (cluster 5/6), tdT+/PDGFRa+/CLU+ (cluster 7), and tdT+/PDGFRa+/HSD11B1 (cluster 8/9) cells in glycerol-injured muscle or NaCl-injected contralateral controls. Data are presented as mean ± SEM. n = 5 per cluster and condition. Significance determined using two-way ANOVA and post hoc Tukey’s test. Significance denoted by an asterisk. NS, not significant.All scale bars represent 200 µm.
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Cell reports
May 10 2022

Fig 3: Clu expression identifies a mineralizing muscle-resident mesenchymal cell population(A and B) Single-cell (A) feature and (B) violin plots for cluster 7-specific gene markers (Clu, Hmcn1) and mineralizing genes (Enpp1, Fam20C).(C) Sorted clusters (C1) tdT+/PDGFRa+ clusters 4–9, (C2) tdT+/PDGFRa+/ADAM12+ cluster 5, (C3) tdT+/PDGFRa+/GAP43+ cluster 6, (C4) tdT+/PDGFRa+/CLU+ cluster 7, (C5) tdT+/PDGFRa+/HSD11B1 clusters 8/9 cells cultured in osteogenic medium for 7 days and then stained with Alizarin red.(D) Quantification of Alizarin red staining relative to tdT+/PDGFRa+ sorted cells. Data are presented as mean ± SEM. n = 4 for each cell type. Significance denoted by an asterisk as calculated by one-way ANOVA.

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GAP43 Antibody

Thermo Fisher Scientific's GAP43 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, and Western Blot. The GAP43 Antibody was generated using GAP43, and Growth Associated Protein 43 as the antigen and it reacts with Homo Sapiens, Human, Mouse, Rat, Bovine, Canine, Chicken/Bird, Drosophila/Arthropod, Horse, Non-Human Primate, and Porcine.

Cell death & disease

Cell reports

Cell reports

GAP43 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

Citations (3)

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GAP43 Antibody from Thermo Fisher Scientific

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