Fig 2: Downregulation of BANCR inactivates MEK, ERK and JNK signaling pathways, but does not affect the p38 mitogen-activated protein kinase pathway. The activity of (A) MEK, (B) ERK, (C) JNK and (D) P38 pathways were determined by western blotting, n=3. Representative images are presented and data are expressed as the mean ± standard deviation, ***P<0.001 compared with the NC group. lncRNA, long non-coding RNA; BANCR, BRAF-activated non-coding RNA; NC, negative control; shRNA, short hairpin RNA; MEK, MAPK/ERK kinase; ERK, extracellular signal-regulated kinase; JNK, janus kinase.

Fig 3: Effects of Sparassis latifolia neutral polysaccharides SLNP on mRNA expression of TLR4 (A), TRAF6 (B), JNK (C), ERK (D), p38 (E), and IRF3 (F) on RAW264.7 macrophage. Compared with blank control group, *p < 0.05, **p < 0.01; Compared with LPS control group, ?p < 0.05, ??p < 0.01.

Fig 4: Effects of Sparassis latifolia neutral polysaccharides SLNP western blot results on protein expression. (A), TLR4 (B), TRAF6 (C), JNK (D), p-JNK (E), ERK (F), p-ERK (G), p38 (H), p-p38 (I), and IRF3 (J) in RAW264.7 macrophage. Compared with blank control group, *p < 0.05, **p < 0.01; Compared with LPS control group, ?p < 0.05, ??p < 0.01.

Fig 5: ASK1/MAPKs signaling pathways are involved in CSO-protected liver injury. (A) The expression of hepatic p-ASK1/ASK1. (B) The expression of hepatic p-JNK/JNK, p-p38/p38, and p-ERK1/2/ERK1/2. *P < 0.05, **P < 0.01, and ***P < 0.001 indicate statistically significant.