Fig 1: ERK and p38 pathways are down-regulated in Spleen Cxcr2-/- neutrophils. (A) NES after GSEA analysis of the transcriptome of spleen neutrophils isolated from Cxcr2-/- versus WT animals according to ERK1 and ERK2 cascade signature. (B) Same analysis for p38-MAPK signature. (C) Heatmap of the significantly regulated genes (p<0.05) of GO-BP ERK1 and ER2 cascade signature. (D) Heatmap of the significantly regulated genes (p < 0.05) of GO-BP p38 MAPK cascade signature. (E) Phospho-p42/44 MAPK ERK1/2 (Thr 202/Tyr 204) was analyzed by flow cytometry in spleen CD11b+ Ly6G+ neutrophils. Left panel: representative Mean of Fluorescence (MFI) of WT (red line) and KO (blue line) Ly6G+ neutrophils. Results are expressed as MFI of phospho-ERK positive neutrophils in the CD11b+ Ly6G+ population and represent the mean ± SEM of at least 6 animals (Mann-Whitney test, *p < 0.05). (F) Phosphorylation of p38MAPK on Thr180/Tyr182 was analyzed by flow cytometry in the same conditions (Mann-Whitney test, ***p < 0.001).