Fig 1: 5-HT7 antagonism disrupts oncogenic signaling in sarcoma cells. (A) Antibody array of HOS osteosarcoma cell lysates collected after treatment for 3 h with 100 nM SB-269970 or a sham control. Red, enhanced expression; green, reduced expression. Two technical replicates of each time point are displayed. (B) HOS cells were treated with 100 nM SB-269970 for 3 h or a sham control. Lysates were collected and subjected to immunoblot using the the KinomeView Profiling kit (Cell Signaling; a set of phospho-motif antibodies that cover a large portion of the kinome and react broadly with serine, threonine, and tyrosine phosphorylation sites mediated by known kinase families. #In the phospho-motif antibody description indicates a phosphorylated amino acid in the consensus motif.