Fig 1: Decreased expression of putative intrafusal specific proteins following Nrg-1 treatment in C2C12 myotubes. Immunofluorescent imaging suggested no clear intrafusal bag myotube preferential expression MyHC3, 8 or 6. Phalloidin (red), DAPI (blue) and MyHC (green). Top to bottom: PN (primary negative), control and Nrg-1 treated representative fluorescent micrographs (scale bar, 200 µm), western blot representative gels and finally MyHC fold change relative to control based on western blots. (A) MyHC3. (B) MyHC8. (C) MyHC6. Western blot lanes represent three biological repeats, n of 3 achieved by repeating on three separate blots, Mean ± SEM, ***, n = 3, p < 0.001.