Fig 1: Ginaton administration rescues Ang II-induced cardiac hypertrophy. Ginaton (300 mg/kg/day) or PBS (internal control) was administered intragastrically to 8-week-old C57BL/6 J mice. Mice were then infused with Ang II (1,000 ng/kg/min) or saline for 14 days. (A) H&E staining of heart tissues in each group (left, n = 4, scale bar = 0.5 mm) and the ratios of heart weight to body weight (HW/BW) and HW to tibial length (HW/TL) (right, n = 8). (B) Heart sections stained with wheat germ agglutinin (WGA) (left, n = 6, scale bar = 20 µm) and quantification of myocyte cross-sectional areas (right, n = 6). (C) qPCR analyses of atrial natriuretic factor (ANF) and brain natriuretic peptide (BNP) mRNA levels (n = 6). (D) Western blot analyses of the expression of phosphorylated (p)-AKT, AKT, p-ERK1/2, ERK1/2, and GAPDH proteins in heart sections (left, n = 4) and the relative protein levels of each (right, n = 4). The results are expressed as means ± SD; n, number of animals per group.