Fig 2: Effect of PDTC on p50 expression in nuclear extracts from whole‐kidney homogenates shown are representative Western blots for p50, with β‐actin as the loading control. Abbreviations: Lew, Lewis; P, PDTC(40 × 2); V, Vehicle.

Fig 3: Effect of PDTC on cortical p50 immunolocalization. The renal cortex is shown from a Lewis rat treated with vehicle (A) and PDTC(40 × 2) (B), and Lewis polycystic kidney (LPK) rats treated with vehicle (C, E) and PDTC(40 × 2) (D, F). Lewis cortices displayed p50 positivity in distal tubule nuclei. In LPK cortices, moderately positive staining was observed in the cytoplasm and nuclei of cystic epithelial cells (CECs) (arrows), and in distal tubule nuclei. There was occasional strongly positive staining in the CECs of focal cysts (E and F). Scale bar = 100 µm.

Fig 4: Effect of PDTC on cortical P‐p105 immunolocalization. Shown are kidney cortices from Lewis rats treated with vehicle (A) and PDTC(40 × 2) (B), and LPK rats treated with vehicle (C, E) and PDTC(40 × 2) (D, F). Lewis cortices displayed P‐p105 positivity in distal tubule nuclei. In LPK kidney cortices, P‐p105 stained strongly in cystic epithelial cell (CEC) cytoplasm and nuclei (arrows). Scale bar = 100 μm.

Fig 5: Disease-specific survival curves for tumour epithelial cell NF-κB p105, vimentin and E-cadherin. NF-κB=nuclear factor-κB.