Fig 1: Effect of total calystegines on the NF-?B/JNK/TLR4 inflammatory pathway triggered by insulin resistance condition in HepG2 cells. a Relative gene expression of c-Jun, NF-?B and Tlr4 factors. b Quantitative representation of total NF- ?B p65 and JNK1/2 proteins. c Quantitative representation of phosphorylated NF- ?B p65(T254) and JNK(Thr183/Tyr185) proteins. d Representative chemiluminescent blots for analysed proteins. e Relative levels of main pro-inflammatory cytokines transcripts. Representative data from three independent experiments are shown ± SD (n = 3). An asterisk (*) indicates a comparison of IR group to untreated healthy cells. A hashtag (#) indicates a comparison of IR group pre-treated with calystegines to IR untreated healthy cells. */#p < 0.05, **/##p < 0.01, ***/###p < 0.001. HepG2_HE: HepG2 healthy untreated cells; HepG2_IR: Insulin resistant HepG2 cells exposed to high concentrations of insulin and glucose. HepG2_IR-H. albus_Caly: Insulin resistant HepG2 cells exposed to high concentrations of insulin and glucose and pre-treated with 250 µg/ml calystegines extracted from Hyoscyamus albus seeds