Fig 1: Immunohistochemistry micrographs of cleft affected tissue and controls. (a) Appearance of numerous IL-7 positive epitheliocytes and few connective tissue cells in cleft affected tissue (IL-7, 200×). (b) Note few-moderate IL-7 immunoreactive epitheliocytes and few connective tissue cells in the control (IL-7, 200×).
Fig 2: (a–h) Immunohistochemical micrographs of patients with recurrent nasal polyps. (a) Recurrent nasal polyp sample with few IL-1a positive structures in the epithelium (blue arrow) and a moderate number of positive structures in the connective tissue (red arrow). IL-1a IMH, X 200; (b) a sample of a recurrent nasal polyp showing moderate IL-4 positive structures in the epithelium (blue arrow) and moderate IL-4 positive structures in the connective tissue (red arrow). IL-4 IMH, X 200; (c) few IL-6 positive structures in the epithelium (blue arrow) and moderate IL-6 positive structures in the connective tissue (red arrow) of a nasal polyp. IL-6 IMH, X 200; (d) few positive structures in the epithelium (blue arrow) and moderate IL-7 positive structures in the connective tissue (red arrow) of a nasal polyp. IL-7 IMH, X 200; (e) nasal polyp sample has few IL-8 positive structures in the epithelium (blue arrow) but a moderate number of positive structures in the connective tissue (red arrow). IL-8 IMH, X 200; (f) a sample of a nasal polyp with few IL-10 positive structures in the epithelium (blue arrow) and moderate-to-numerous numbers of IL-10 positive cells in the connective tissue (red arrow). IL-10 IMH, X 200; (g) few IL-12 positive structures in the epithelium (blue arrow) and numerous positive cells in the connective tissue (red arrow) of a nasal polyp. IL-12 IMH, X 200; (h) occasional Ki-67 positive structures in the epithelium (blue arrow) and few positive cells in the connective tissue (red arrow) of a nasal polyp. Ki-67 IMH, X 200.
Fig 3: Healthy nasal mucosa presenting numerous number of IL-1 positive superficial cells (arrow) (IL-1 IMH, ×200) (a), abundancy of IL-4 positive cells (arrows) in superficial epithelium (IL-4 IMH, ×200) (b), numerous number of IL-6 positive cells (arrow) in superficial epithelium (IL-6 IMH, ×200) (c), and numerous number of IL-7 positive epithelial cells (arrow) in healthy nasal mucosa. (IL-7 IMH, ×200) (d).
Fig 4: Micrographs of gallbladder in children with cholecystitis and in controls. (a) Note numerous IL-7 positive epitheliocytes and moderate number of IL-7 positive cells in connective tissue. IL-7 IMH, ×200; (b) note numerous IL-7 positive cells only in the epithelium of control tissue. IL-7 IMH, ×200; (c) few to moderate number of IL-8 positive epithelial and connective tissue cells of the patient. IL-8 IMH, ×200; (d) control tissue containing only few IL-8 positive cells. IL-8 IMH, ×200.
Fig 5: (a–d) Immunohistochemical micrographs of inpatients with nasal polyps and control subjects. (a) 42-year-old male with healthy nasal mucosa. Numerous IL-6 positive structures in the control sample epithelium and few positive structures in connective tissue. IL-6 IMH, X 250; (b) 30-year-old female with CRSwNP. Few to moderate IL-6 positive structures in epithelium and moderate to numerous IL-6 positive structures in connective tissue of a nasal polyp. IL-6 IMH, X 250; (c) 42-year-old male with healthy nasal mucosa. Numerous IL-7 positive structures in epithelium of a control sample with few to moderate positive structures in connective tissue. IL-7 IMH, X 250; (d) 33-year-old male with CRSwNP. Few to moderate positive structures in epithelium and moderate to numerous IL-7 positive structures in the connective tissue of a nasal polyp. IL-7 IMH, X 250.
Supplier Page from Biorbyt for IL7 antibody
Application Dilutions: WB=1:500-2000, ELISA=1:5000-10000