Fig 1: Lysozyme induces GOT overexpression by TLR4 activation. (A, B) Representative western blot images showing effects of lysozyme-mediated activation of TLR4 on GOT expression in (A) SH-SY5Y human neuronal cells treated with lysozyme (n=4 independent experiments, ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test p<0.005) (B) mice treated with lysozyme (n=4 independent experiments, ordinary one-way ANOVA p<0.005). (C) Representative western blot images showing the effects of lysozyme on the expression of two different GOT isoforms in SH-SY5Y cells (n=4 independent experiments, t-tests p<0.005). (D) Specific inhibition of GOT2 isoform affects lysozyme-mediated pain sensitization in mice (n=10 mice per group, two-way ANOVA followed by Sidak’s multiple comparison p<0.005). (E) Effect of lysozyme treatment on neuronal glutamate amount in GOT2-siRNA-treated SH-SY5Y cells (n=4 independent experiments, p<0.005 versus Sc-siRNA treatment, ordinary one-way ANOVA).