Fig 2: WBM-alleviated hypoxia triggers ferroptosis and ER stress in microglial BV-2 cells via Gpx4/SLC7A11 and CHOP/Bip-dependent pathways, respectively. (a) The upper panel indicates expression changes of Gpx4/SLC7A11 proteins. The lower panel indicates the quantitative results of assessing specific proteins using Image J. (b) The upper panel indicates expression changes of CHOP and Bip proteins. The lower panel indicates the quantitative results of assessing specific proteins using Image J. (c) Expression changes of Gpx4 and SLC7A11 proteins in presence or absence of 20 µM erastin. (d) Expression changes of CHOP and Bip proteins in presence or absence of 1 uM thapsigargin (Thg). Three independent replicates were used to calculate the standard deviation (n = 3). *P < 0.05. H, hypoxia. H/WBM, in presence of 30 µM WBM under hypoxia.

Fig 3: Hypoxia induces ferroptosis via the Gpx4/SLC7A11 downregulation, decreases cell survival, and induces ER stress via CHOP/Bip upregulation. (a) Changes in lipid ROS levels of control (without 2 µM C11-BODIPY staining), normoxia, and hypoxia cells (with 2 µM C11-BODIPY staining). (b) Mean fluorescence intensity is used to measure lipid ROS levels. (c) WST-1 measures the viability of cells in the indicated groups. (d) The upper panel indicates expression changes of Gpx4 and SLC7A11 proteins. The lower panel indicates the quantitative results of assessing specific proteins using ImageJ. (e) The upper panel indicates the expression changes of CHOP and Bip proteins. The lower panel indicates the quantitative results of assessing specific proteins using Image =J. Three independent replicates were used to calculate the standard deviation (n = 3). *P < 0.05. N, normoxia; H, hypoxia.