Fig 2: The expression and methylation levels closely related to growth (NNMT, GALP, IGF-1, IGFBP2) in the liver of piglets fed diets containing DON-contaminated corn (n = 7). (A) The mRNA levels of NNMT, GALP, IGF-1 and IGFBP2 were detected by qPCR. (B) The protein expression of NNMT, GALP and IGF-1 was detected by Western blot. (C) The methylation status of CpG sites in gene promoter region was detected by methylation-specific PCR (MSP). M for methylation, U for unmethylation. (D) The methylation levels in gene promoter regions were detected by bisulfite sequencing (BSP). The blue dots for the CpG sites of methylation, and the white dots for the unmethylated CpG sites. *P < 0.05, **P < 0.01 versus 0 mg/kg group. CpG = cytosine-phosphate-guanine; GALP = galanin-like peptide; NNMT = nicotinamide N-methyltransferase; IGF-1 = insulin-like growth factor 1; IGFBP2 = insulin-like growth factor-binding protein 2.