Fig 1: The m6A modification was involved in renal ischemia-reperfusion injury (IRI) and the hypoxia and reoxygenation (H/R) response in mouse renal tubular epithelial cells (mRTECs).a m6A dot blot assessed m6A mRNA methylation at different time in mouse IRI model. b Real-time quantitative PCR (RT-qPCR) analysis of m6A regulators: AlkB homolog 5 (Alkbh5), obesity-associated gene (Fto), methyltransferase-like protein 3 (Mettl3), methyltransferase-like protein 14 (Mettl14), and Wilms’ tumor 1-associating protein (Wtap) in IRI model. n = 3, 4, 4, 3, 3 for Sham, 12 h, 24 h, 48 h, and 120 h groups, respectively. Each data point represent one animal. Unpaired two-tailed Student’s t-test. c Western blot assay measured the protein levels of m6A regulators (ALKBH5, FTO, METTL3, METTL14, and WTAP) in IRI kidneys. Three biological repeated immunoblots have been performed. d Representative immunofluorescent (IF) staining of ALKBH5 (red), tetragonolobus lectin (LTL) (green), and DAPI (blue) in kidney biopsies from sham or IRI mice. (Magnification: ×400, Scale bar: 50 μm) (n = 5 mice for all groups). e m6A dot blot assessed m6A mRNA methylation of H/R model in mRTECs. f RT-qPCR analysis of m6A regulators (Alkbh5, Fto, Mettl3, Mettl14, and Wtap) in H/R model in mRTECs. n = 4, 5, 5 biologically independent cells for Control, H24R6, H24R12 groups, respectively. Unpaired two-tailed Student’s t-test. g Western blot analysis of m6A regulators (ALKBH5, FTO, METTL3, METTL14, and WTAP). Three biological repeated immunoblots have been performed. Data are showed as means ± SD.