Fig 1: The Novel rat model of Xdh knockout in Dahl SS background(A) Schematic showing the location of the deletion in the Xdh gene on rat chromosome 6.(B) Western blotting analysis of Xdh expression in the kidney cortex of the SSXdh+/-, SSXdh+/+, and SSXdh-/- rats (Each band = one rat).(C) Representative images of immunostaining of Xdh protein in the kidneys of the SSXdh+/+, SSXdh+/-, and SSXdh-/- rats (N = 4 per group).(D–J) Survival probability of the SSXdh+/+ (N = 37), SSXdh+/- (N = 57), and SSXdh-/- (N = 28) rats in the first 6 weeks of their lives (For separate analyses on male and female survival see Figure S1A). (E) Total Body Weight (TBW), (F) Sum weight of both kidneys (G) Sum weight of kidneys/TBW ratio (H) Heart weight/TBW ratio, (I) Uricemia and (J) Uricosuria of the SSXdh+/+, SSXdh+/-, and SSXdh-/- male rats (For female see Figure S1B). All data are from male 6-week-old rats except for Figure 1D which includes both males and females. ND = Not Detected (below the level of detection), in cases of ND, the lowest possible value of detection was used for statistical analyses. For E-J, N = the number of shapes; NS- not significant, * =0.05, ** =0.01, *** =0.001, **** =0.0001. Data are represented as mean ± SEM.