Fig 1: Expression of membrane proteins involved in reticulocyte maturation. Red cell membranes from heterozygous SAO and control peripheral blood samples were prepared, separated by SDS-PAGE and immunoblotted. The antibody directed against CD44 was an in-house rabbit polyclonal antibody. Commercial antibodies were used to detect actin (ab6276), calreticulin (ab2907); CD147 (ab108308), LAMP-2 (ab25631) and transferrin receptor (TfnR) (ab84036); all from Abcam, Cambridge, United Kingdom. An in-house monoclonal antibody was used to detect beta-spectrin (BRAC65). SAO het: Membranes prepared from the heterozygous father of the homozygous SAO child. LC: Loading control.
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