Fig 1: CXCR4 gene and protein expressions in HRECs. (A) Semi-quantitative RT-PCR to evaluate mRNA expression of CXCR4. HRECs were harvested to extract total RNAs. RT-PCR was performed using the obtained total RNAs. (B) Western blotting was used to evaluate protein expression of CXCR4. HRECs were harvested to extract the total protein (n=3). (C) IgG isotype control performed by flow cytometry. (D) Flow cytometry to examine the expression of CD106 and CD54 in HRECs. CXCR, chemokine (C-X-C motif) receptor; HREC, human retinal endothelial cell; RT-PCR, reverse transcription-PCR.