Fig 1: Atp11b-KO mice display aging pathology. (A) SA-β-Gal staining of the mouse hippocampus. (B) Quantitation of the SA-β-Gal-positive cells in the CA3 region. (C) Quantitation of SA-β-Gal expression in the DG region. Scale bars: 100 μm; for each group of mice, n = 4. (D) p-HistoneH2AX (green) fluorescence staining of the mouse hippocampus. (E) Average fluorescence intensity of p-HistoneH2AX in the mouse hippocampus. (F) p16INK4 (green) fluorescence staining in the mouse hippocampus. (G) Mean fluorescence intensity of p16INK4 in the mouse hippocampus. Scale bars: 200 μm; for each group of mice, n = 3. The mice were 12 months old; data are expressed as the mean ± SEM. Student’s t-test, * p < 0.05, ** p < 0.01, *** p < 0.001.