Fig 2: The expression of CRGs in CRC cell lines and tissues. (A-J) The mRNA relative expression of each CRG in 5 CRC cell lines and normal colon cell line. The asterisks illustrated the statistical p-value. (K) Western blotting results of PDHA1 protein levels in five CRC cell lines and the normal colon cell line. (L) PDHA1 was downregulated in colorectal cancer tissues compared to normal tissues, as examined by immunohistochemistry. (M) Kaplan–Meier analysis of the disease-free survival rate of CRC patients, which is stratified according to the expression of PDHA1.

Fig 3: Experimental verification in the UC mouse model. (A) Hematoxylin and eosin-stained sections depicting mouse colonic mucosa in the control and DSS groups. (B) DAI scores increased after acute administration of DSS. (C) Histological inflammation scores increased after DSS treatment. (D–F) The expression levels of DLAT, DLD, and PDHA1 were detected by using real-time quantitative polymerase chain reaction. (G, H)The expression levels of DLAT, DLD, and PDHA1 were detected by Western blot analysis in the UC mouse model. (*p < 0.05; **p < 0.01; ***p < 0.001;****p < 0.0001)