Fig 1: FTO-mediated m6A modification of OGDHL mRNA in ccRCC.A Heatmap of differential gene expression in ccRCC tissues and normal tissues. B mRNA levels of m6A-regulated enzymes in ccRCC and paracancerous normal tissues (Tissues are derived from clinical, surgical samples). C Overall mRNA m6A levels in ccRCC and normal tissue samples were determined by RNA m6A dot blot. D Overall mRNA m6A levels of ccRCC cell line versus control 293 cell line. E A GSEA correlation chart based on the TCGA-KIRC database with FTO expression as the premise for screening. FDR < 25% and p < 0.05 was considered statistically significant. F qRT-PCR was used to verify the mRNA levels of FTO knockdown or overexpressed. G Western blot analysis was used to verify the protein levels of FTO knockdown or overexpressed. H Knockdown of FTO promoted m6A methylation in OGDHL mRNA by m6A MeRIP analysis. I Wild-type OGDHL 3'UTR and OGDHL 3'UTR with mutations at the m6A consensus sequence were cloned into a luciferase reporter gene. Mutations in the m6A modified region were generated by substituting thymine for adenosine. J Relative luciferase activity of wild-type and mutant OGDHL 3'UTR reporter vectors in FTO knockdown or overexpressing A498 cells.