Fig 2: USP10 activated TSC2 and JNK1 signaling pathways in HepG2 cells.HepG2 cells overexpressing USP10 were treated with PA (312.5 µM) for 24 h. Protein expression was assayed by western blotting. (A) Protein expression of tuberous sclerosis complex-2 (TSC2), mammalian target of rapamycin (mTOR), p-mTOR, ribosomal protein S6 kinase (S6K), and p-S6K. Data are means±SD, n=3 (**p<0.01). (B) Expression of C-jun N-terminal protein kinase-1 (JNK1), VPS34, ATG14, Bcl2, and p-Bcl2. Data are means±SD, n=3 (*p<0.05; **p<0.01; NS, not significant). ATG, autophagy target gene; JNK, C-jun N-terminal protein kinase; mTOR, mammalian target of rapamycin; S6K, ribosomal protein S6 kinase; TSC, tuberous sclerosis complex; USP, ubiquitin-specific peptidase; VPS34, phosphatidylinositol 3-kinase.