Anti-RhoA antibody (mouse MAb) + control ARH03-A from Cytoskeleton, Inc.

Product Specs
ItemAnti-RhoA antibody (mouse MAb) + control
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberARH03-A
ApplicationsWB, ICC
Reactivityhuman, mouse, rat
HostMouse
ClonalityMonoclonal
Quantity2 x 50 µg
TargetAnti-RhoA
Conjugate/TagUnconjugated
FormatPurified
Brand/TrademarkCytoskeleton, Inc

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) Oncogenic BRAF induces whole-genome doubling through suppression of cytokinesisNature communicationsJuly 15, 2022Darp R, Vittoria MA, Ganem NJ, Ceol CJ.TUBULIN 1:1000 (2144S, Cell Signaling); BRAFV600E 1:1000 (E19290, Spring Bioscience); RHOA(GTP) 1:500 (ARH03, Cytoskeleton); Total RHOA, 1:500 (ARH05, Cytoskeleton), RAC1 1:500 (ARC03, Cytoskeleton); p-MPS1 T676

(2) Cell-in-cell structure mediates in-cell killing suppressed by CD44Cell discoveryApril 19, 2022Su Y, Huang H, Luo T, Zheng Y, Fan J, Ren H, Tang M, Niu Z, Wang C, Wang Y, et al.or 1:1000 for WB; BD Biosciences, #610253), anti-RhoA (1:200 for IF or 1:1000 for WB; Cytoskeleton, #ARH03), anti-Rac1 (1:1000; Proteintech, #24072-1-AP), anti-pMLC (1:200 for IF or 1:1000 for WB; CST, #3671),

(3) Dynamic interplay of two molecular switches enabled by the MEK1/2-ERK1/2 and IL-6-STAT3 signaling axes controls epithelial cell migration in response to growth factorsThe Journal of biological chemistryOctober 1, 2021Qin L, Cao X, Kaneko T, Voss C, Liu X, Wang G, Li SS.,BD Biosciences,612020 Mouse monoclonal anti-PI3K,Millipore,1981549 Mouse monoclonal anti-RhoA,Cytoskeleton,ARH03 Rabbit polyclonal anti-Rac1,Santa Cruz,Sc-217 Mouse monoclonal anti-VaV2,Santa Cruz,Sc-271442 Rabbit

(4) Cx43 and Associated Cell Signaling Pathways Regulate Tunneling Nanotubes in Breast Cancer CellsCancersSeptember 29, 2020Tishchenko A, Azorín DD, Vidal-Brime L, Muñoz MJ, Arenas PJ, Pearce C, Girao H, Ramón Y Cajal S, Aasen T.vimentin (Santa Cruz Biotechnologies; #sc-6260; 1:1000), and RhoA (Cytoskeleton Inc., Denver, CO, USA; #ARH03; 1:1000). The secondary antibodies were rabbit anti-mouse horseradish peroxidase and goat anti-rabbit

(5) Functional Genomic Screening Independently Identifies CUL3 as a Mediator of Vemurafenib Resistance via Src-Rac1 Signaling AxisFrontiers in OncologyApril 3, 2020Marion Vanneste et al.Primary antibodies "RAC1 (#610651, BD Transduction)" "CDC42 (#2462, Cell Signaling)" "RHOA (#ARH03, Cytoskeleton)" "RHOC (#3430S, Cell Signaling)" "RHOGDI (#2564S, Cell Signaling)" "p44/42 MAPK (ERK1/2)

(6) Protein prenylation restrains innate immunity by inhibiting Rac1 effector interactionsNature communicationsSeptember 4, 2019Akula MK, Ibrahim MX, Ivarsson EG, Khan OM, Kumar IT, Erlandsson M, Karlsson C, Xu X, Brisslert M, Brakebusch C, et al.Millipore); Cdc42 (sc-87), Iqgap1 (sc-10792), and Tiam1 (sc-376021, Santa Cruz Biotechnology); and RhoA (ARH03, Cytoskeleton), using the Dynabeads Protein G Immunoprecipitation Kit (10007D, Thermo Fisher Scientific).

(7) Contributions of the RhoA guanine nucleotide exchange factor Net1 to polyoma middle T antigen-mediated mammary gland tumorigenesis and metastasisBreast Cancer ResearchDecember 1, 2018Yan Zuo, Arzu Ulu, Jeffrey T. Chang, Jeffrey A. Frost(sc-138), anti-Src (sc-8056), and normal rat IgG (sc-2026) (SantaCruz Biotechnology); and anti-RhoA (ARH03) (Cytoskeleton, Inc.). Primary tumor cell isolation and mammary gland transplantation Individual mammary

(8) Withdrawal from repeated morphine administration augments expression of the RhoA network in the nucleus accumbens to control synaptic structureJournal of NeurochemistryOctober 1, 2018Michael E. Cahill, Caleb J. Browne, Junshi Wang, Peter J. Hamilton, Yan Dong, Eric J. NestlerMRTF-A (MAL) (Cat# sc-21558; RRID: AB_2142498). RhoA antibody was purchased from Cytoskeleton (Cat# ARH03-A; RRID: AB_10708069). Antibody validations are available from each company. Cell fractionation The

(9) Fam65b Phosphorylation Relieves Tonic RhoA Inhibition During T Cell MigrationFrontiers in ImmunologySeptember 11, 2018Laura Megrelis, Elyas El Ghoul, Federica Moalli, Margaux Versapuech, Shamir Cassim, Nora Ruef, Jens V. Stein, Marianne Mangeney, Jérôme Delonstaining for total RhoA was carried out in parallel with a similar protocol using an anti-RhoA antibody (# ARH03, Cytoskeleton). Microscopy Transfected live CEM cells stained with DAPI were analyzed by fluorescence microscopy

(10) EGFL7 enhances surface expression of integrin α₅β₁ to promote angiogenesis in malignant brain tumorsEMBO molecular medicineSeptember 1, 2018Dudvarski Stanković N, Bicker F, Keller S, Jones DT, Harter PN, Kienzle A, Gillmann C, Arnold P, Golebiewska A, Keunen O, et al.(#ACD03, 1:250, Cytoskeleton), mouse anti‐Rac1 (#ARC03, 1:500, Cytoskeleton), or mouse anti‐RhoA (#ARH03, 1:500, Cytoskeleton). Epigenetic analyses Epigenetic analyses, i.e., Aza and PBA cell treatment,

(11) A RhoA-YAP-c-Myc signaling axis promotes the development of polycystic kidney diseaseGenes & developmentJune 1, 2018Cai J, Song X, Wang W, Watnick T, Pei Y, Qian F, Pan D.Cell Signaling, 13619), and anti-c-Myc (1:1000; Abcam, ab32072) and mouse anti-RhoA (1:500; Cytoskeleton, Inc., ARH03) and anti-Actin (1:20,000; Millipore, MAB1501R). Human renal tissue analysis Formalin-fixed paraffin-embedded

(12) Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cellseLifeMay 29, 2018Stefanie Giera et al.Scientific Corporation, WESTBURY, NY; Cat #OBT0030S, 1:100), mouse anti-RhoA (Cytoskeleton, Denver, CO; Cat# ARH03-A, 1:500), mouse anti-CDK2 (Santa Cruz, Dallas, TX; Cat #sc-6248, 1:1000), mouse anti-β-actin (Sigma,

(13) NFkB hyperactivation causes invasion of esophageal squamous cell carcinoma with EGFR overexpression and p120-catenin down-regulationOncotargetFebruary 16, 2018Heather L. Lehman, Michal Kidacki, Joshua I. Warrick, Douglas B. StairsCell Signaling Technology. The antibody against Vav2 (1:5000; ab52640) was from Abcam. The antibody against RhoA (1:500; #ARH03) was from Cytoskeleton, Inc. β-actin (1:10,000; #A5316), used as a loading control for immunoblotting,

(14) Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machineryeLifeDecember 22, 2017Chang TY, Chen C, Lee M, Chang YC, Lu CH, Lu ST, Wang DY, Wang A, Guo CL, Cheng PL.Rac1-GTP (26903) was from NewEast Biosciences (King of Prussia, PA). Monoclonal antibodies to RhoA (ARH03), Rac1 (ARC03) and Cdc42 (ACD03) were from Cytoskeleton Inc. (Denver, CO). Pharmacological reagents:

(15) Induced cortical tension restores functional junctions in adhesion-defective carcinoma cellsNature CommunicationsDecember 1, 2017Shoko Ito, Satoru Okuda, Masako Abe, Mari Fujimoto, Tetsuo Onuki, Tamako Nishimura, Masatoshi Takeichiproduced by BD; rabbit anti-Par3 (Cat#07-330, 1:50 for IF) produced by Millipore; mouse anti-RhoA (Cat#ARH03, 1:100 for WB) produced by Cytoskeleton. Uncropped western blots obtained using these antibodies are

(16) Glucocorticoid therapy regulates podocyte motility by inhibition of Rac1Scientific reportsJuly 27, 2017McCaffrey JC, Webb NJ, Poolman TM, Fresquet M, Moxey C, Zeef LAH, Donaldson IJ, Ray DW, Lennon R.Proteintech), phosphorylated GR (serine 211) (4161, Cell Signaling), Rac1 (610651, BD Biosciences), RhoA (ARH03, Cytoskeleton), GAPDH (G8795, Sigma-Aldrich). Secondary antibodies conjugated to Alexa Fluor 680 (A21109,

(17) NRF2 promotes breast cancer cell proliferation and metastasis by increasing RhoA/ROCK pathway signal transductionOncotargetNovember 8, 2016Zhang C, Wang HJ, Bao QC, Wang L, Guo TK, Chen WL, Xu LL, Zhou HS, Bian JL, Yang YR, et al.3674, 3285, 8556 and 13826, respectively). Mouse-anti-RhoA was purchased from Cytoskeleton (Cat No. ARH03, CA), and mouse-anti-actin was purchased from ProteinTech (Cat No. 60008-1-Ig, CA). Cell proliferation

(18) The X-Linked Autism Protein KIAA2022/KIDLIA Regulates Neurite Outgrowth via N-Cadherin and δ-Catenin SignalingeNeuroSeptember 1, 2016Gilbert J, Man HY.BD Biosciences; cat. # 611536, RRID: AB_398994), mouse anti-RhoA (1:500 for WB; Cytoskeleton, Denver, CO; cat. # ARH03-A, RRID: AB_10708069), mouse HDAC1 (1:1000 for WB; Cell Signaling Technology, Danvers, MA; cat. # 5356P,

(19) RhoA activity increases due to hypermethylation of ARHGAP28 in a highly liver-metastatic colon cancer cell lineBiomedical ReportsMarch 1, 2016KAZUHIKO KASUYA, YUICHI NAGAKAWA, YUICHI HOSOKAWA, YATSUKA SAHARA, CHIE TAKISHITA, TETSUSHI NAKAJIMA, YOSUKE HIJIKATA, RYOKO SOYA, KENJI KATSUMATA, AKIHIKO TSUCHIDAFollowing blocking, the membrane was reacted with an anti-RhoA monoclonal antibody as a primary antibody (ARH03; Cytoskeleton, Inc.), and chemiluminescence emitted by the target band using electrochemiluminescence

(20) Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm BirthJournal of immunology (Baltimore, Md. : 1950)October 1, 2015Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, et al.electrotransferred on polyvinylidene difluoride membranes, blocked, and incubated with an anti-RhoA mAb (ARH03; Cytoskeleton) overnight at 4˚C. The membrane was then incubated with a HRP-conjugated anti-mouse secondary

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Anti-RhoA antibody (mouse MAb) + control from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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Anti-RhoA antibody (mouse MAb) + control from Cytoskeleton, Inc.

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