Anti-tubulin polyAb pan specific (sheep) + control ATN02-B from Cytoskeleton, Inc.

Product Specs
ItemAnti-tubulin polyAb pan specific (sheep) + control
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberATN02-B
ApplicationsWB, IC, ELISA, IP
Reactivityyeast, fungi, and animal species
HostSheep
TargetAnti-tubulin polyAb pan
FormatPurified
Conjugate/TagUnconjugated
Quantity3 x 100 µg
Brand/TrademarkCytoskeleton, Inc

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) Abnormal centriolar biomarker ratios correlate with unexplained bull artificial insemination subfertility: a pilot studyScientific ReportsOctober 26, 2023Katerina A. Turner, Luke Achinger, Dong Kong, Derek F. Kluczynski, Emily Lillian Fishman, Audrey Phillips, Barbara Saltzman, Jadranka Loncarek, Bo R. Harstine, Tomer Avidor-Reisscatalog number, batch number",Dilution,Role,RRID Tubulin,"Sheep anti-tubulin, Cytoskeleton, Inc., ATN02",1:600,Primary antibody,AB_10708807 Acetylated tubulin,"Mouse anti-Acetylated tubulin, Sigma Aldrich,

(2) CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoaMicroPubl BiolSeptember 25, 2023Katerina A. Turner, Drew L. Caswell, Bryce M. McGrady, Andrea Pietras-Allen, Jessica Sedlak, Cassandra Nathan, Surya Parasuraman, Aidan P. McGann, Farzeen M. Fazili, Jonathan R. Bell, et al.demonstrated in (Turner, Katerina A., et al. 2022) . Anti-Tubulin made in Sheep (Cytoskeleton, Inc. ATN02). The specificity of the antibody was demonstrated in the references (Piroli et al. 2014; Lobert et

(3) The GCN2/eIF2αK stress kinase regulates PP1 to ensure mitotic fidelityEMBO reportsAugust 3, 2023Vilte Stonyte, Maria Mastrangelopoulou, Romy Timmer, Lilian Lindbergsengen, Marina Vietri, Coen Campsteijn, Beáta GrallertSystems 708210); rabbit pericentrin 1:400 (Abcam ab4448); sheep α/β‐tubulin 1:500 (Cytoskeleton Inc ATN02); rabbit phospho‐Aurora A(T288)/B(T232)/C(T198) 1:200 (Cell Signaling #2914); human CREST 1:2,000 (Fitzgerald Industries

(4) Vesicle-mediated transport of ALIX and ESCRT-III to the intercellular bridge during cytokinesisCellular and Molecular Life SciencesAugust 1, 2023Sascha Pust, Andreas Brech, Catherine Sem Wegner, Harald Stenmark, Kaisa HaglundKIF5B (1:100, Abcam #ab151558), KLC1 (1:100, Santa Cruz #sc25735) and tubulin (1:200, Cytoskeleton #ATN02) were used as primary antibodies, and the assays were performed as described in the manufacturer’s manual.

(5) A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituentsMolecular biology of the cellNovember 1, 2022LaJoie D, Turkmen AM, Mackay DR, Jensen CC, Aksenova V, Niwa M, Dasso M, Ullman KS.α-tubulin (Abcam; ab6160); chicken α-tubulin (Synaptic Systems; 302 206); sheep α-tubulin (Cytoskeleton; ATN02); followed by AlexaFluor conjugated secondary antibodies (ThermoFisher). Coverslips were mounted on

(6) PHLPP1 regulates CFTR activity and lumen expansion through AMPKDevelopment (Cambridge, England)October 15, 2022Lobert VH, Skardal ML, Malerød L, Simensen JE, Algra HA, Andersen AN, Fleischer T, Enserink HA, Liestøl K, Heath JK, et al.provided by Thomas C. Kaufman (Indiana University, Bloomington, USA). Sheep anti-α/β-tubulin (1:200; ATN02) was purchased from Cytoskeleton. Rabbit anti-Bazooka (1:500) was obtained from Tony Harris (University

(7) H-ABC tubulinopathy revealed by label-free second harmonic generation microscopyScientific ReportsAugust 24, 2022Milvia Alata, Valeria Piazza, Carla Jaramillo-Restrepo, Jose R. Eguibar, Carmen Cortes, Victor H. Hernandezwere marked with anti-APC antibody [CC-1] (ab16794, abcam) and polyclonal anti- Alpha/Beta-tubulin (ATN02, Cytoskeleton), Anti-Neurofilament 200 (N4142, Sigma), anti-NeuN (EPR12763, Abcam) or anti-Olig2 antibody (EPR2673

(8) Cucurbitacins elicit anti-platelet activity via perturbation of the actin cytoskeleton and integrin functionThrombosis and HaemostasisJuly 30, 2022Neline Kriek, Sophie H. Nock, Tanya Sage, Badrija Khalifa, Alexander P. Bye, Joanne L. Mitchell, Steven Thomson, Mark G. McLaughlin, Sarah Jones, Jonathan Martin Gibbins, et al.United Kingdom). β-actin (C4) antibody was purchased from Santa Cruz (# sc47778), tubulin antibodies (#ATN02) from Cytoskeleton Inc. (Denver, United States) and β-tubulin (# PAS-16863) from Invitrogen and platelet

(9) Centrosome-dependent microtubule modifications set the conditions for axon formationCell reportsApril 19, 2022Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, et al.anti-Tryosinated tubulin (YL1/2), 1:400 (Abcam ab6160); sheep anti-tubulin (total), 1:400 (Cytoskeleton ATN02). Secondary – goat anti-guinea pig IgG AF 647, 1:1000 (Invitrogen A21450); donkey anti-mouse IgG AF

(10) Tubulin posttranslational modifications modify the atypical spermatozoon centriolemicroPublication biologyJanuary 1, 2022Turner KA, Kluczynski DF, Hefner RJ, Moussa RB, Slogar JN, Thekkethottiyil JB, Prine HD, Crossley ER, Flanagan LJ, LaBoy MM, et al.al. 2016; Liu et al. 2020; Amargant et al. 2021). Anti-Tubulin made in Sheep (Cytoskeleton, Inc., ATN02) (Diluted 1 to 600 in cattle and 1 to 300 up to 600 in human sperm). The specificity of the antibody

(11) α-TubK40me3 is required for neuronal polarization and migration by promoting microtubule formationNature CommunicationsDecember 1, 2021Xuan Xie, Shaogang Wang, Mingyi Li, Lei Diao, Xingyu Pan, Jijun Chen, Weiguo Zou, Xu Zhang, Wenfeng Feng, Lan Baodark, final immunostaining was processed using sheep antibody against tubulin (1:500; Cytoskeleton ATN02) or rat antibody against tyrosinated tubulin (1:1000; Abcam ab6160). Electron microscopy For transmission electron microscopy

(12) Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3AeLifeNovember 23, 2021Creighton BA, Afriyie S, Ajit D, Casingal CR, Voos KM, Reger J, Burch AM, Dyne E, Bay J, Huang JK, et al.anti-NeuN (1:1,000, clone A60, #ABN90P) from Millipore-Sigma; sheep anti-alpha/beta tubulin (1:200, #ATN02) from Cytoskeleton; chicken anti-GFP (1:1000, #GFP-1020) from Aves; rabbit anti-Olig2 (1:200, # 13999–1-AP)

(13) STING nuclear partners contribute to innate immune signaling responsesiScienceSeptember 24, 2021Charles R. Dixon, Poonam Malik, Jose I. de las Heras, Natalia Saiz-Ros, Flavia de Lima Alves, Mark Tingey, Eleanor Gaunt, A. Christine Richardson, David A. Kelly, Martin W. Goldberg, et al.GTU-88",Merck,Cat# T6557; RRID:AB_477584 Sheep polyclonal anti-alpha tubulin,Cytoskeleton Inc.,Cat# ATN02; RRID:AB_10708807 "Mouse monoclonal anti-SYNCRIP, 7A11.2",Millipore,Cat# MAB11004; RRID:AB_10806916 Rabbit

(14) MLKL trafficking and accumulation at the plasma membrane control the kinetics and threshold for necroptosisNature communicationsJune 19, 2020Samson AL, Zhang Y, Geoghegan ND, Gavin XJ, Davies KA, Mlodzianoski MJ, Whitehead LW, Frank D, Garnish SE, Fitzgibbon C, et al.3493; 1:1000) mouse anti-Vti1a (BD Biosciences; Cat#6112200), sheep anti-tubulin (Cytoskeleton, Inc. # ATN02), mouse anti-actin (Abcam #ab14128; used in Fig. 7a), anti-β-Actin (Sigma #A1987; used in Supplementary

(15) Identification of the Repressive Domain of the Negative Circadian Clock Component CHRONOInternational Journal of Molecular SciencesApril 2, 2020Yu Yang, Ning Li, Jiameng Qiu, Honghua Ge, Ximing Qinrabbit antibody against GFP-tag (D110008, BBI, Shanghai, China) and sheep antibody against Tubulin (ATN02, Cytoskeleton, Denver, United States) were subjected to Western blot according to the manufacturer’s protocol.

(16) hnRNPDL Phase Separation Is Regulated by Alternative Splicing and Disease-Causing Mutations Accelerate Its AggregationCell ReportsJanuary 28, 2020Cristina Batlle, Peiguo Yang, Maura Coughlin, James Messing, Mireia Pesarrodona, Elzbieta Szulc, Xavier Salvatella, Hong Joo Kim, J. Paul Taylor, Salvador Venturaanti GAPDH,Santa Cruz,Cat#sc47724; RRID: AB_627678 Sheep polyclonal anti tubulin,Cytoskeleton Inc,Cat#ATN02; RRID:AB_10709401 Mouse monoclonal anti C23,Santa Cruz,Cat#sc-8031; RRID: AB_670271 ,, Bacterial

(17) Symmetrically dispersed spectroscopic single-molecule localization microscopyLight, science & applicationsJanuary 1, 2020Song KH, Zhang Y, Brenner B, Sun C, Zhang HF.microtubules and mitochondria, the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1, sc-17764, Santa Cruz). After washing with washing

(18) ATAT1-enriched vesicles promote microtubule acetylation via axonal transportScience AdvancesDecember 18, 2019Aviel Even et al.no.,WB,IF,ELISA α-Tubulin,Sigma-Aldrich,T9026,1:5000,,"1:2,000" α-Tubulin*,DSHB,12G10,1:500,, α/β-Tubulin,Cytoskeleton,ATN02-A,,1:150, Acetylated α-tubulin,Sigma-Aldrich,T7451,"1:15,000","1:15,000", Polyglutamylated tubulin,AdipoGen,AG-25B-0030,"1:10,000",, Glutamylated

(19) The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for MyelinationCellSeptember 19, 2019Fu MM, McAlear TS, Nguyen H, Oses-Prieto JA, Valenzuela A, Shi RD, Perrino JJ, Huang TT, Burlingame AL, Bechstedt S, et al.monoclonal",ThermoFisher Scientific,Cat#MA1–80017 "Anti-α/β-tubulin, sheep polyclonal",Cytoskeleton,Cat#ATN02 "Anti-γ-tubulin (Clone DQ-19), rabbit polyclonal",Sigma,Cat#T3195 "Anti-γ-tubulin (Clone GTU-88),

(20) Chlorpyrifos oxon promotes tubulin aggregation via isopeptide cross-linking between diethoxyphospho-Lys and Glu or Asp: Implications for neurotoxicityJournal of Biological ChemistryAugust 1, 2018Lawrence M. Schopfer, Oksana Lockridgediethoxyphosphotyrosine (55) or with a sheep polyclonal that targeted tubulin (Cytoskeleton, catalog number ATN02) in TBST plus 5% nonfat dry milk, for 18 h at 6 °C. After washing with TBST, the membrane was hybridized

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Anti-tubulin polyAb pan specific (sheep) + control from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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Anti-tubulin polyAb pan specific (sheep) + control from Cytoskeleton, Inc.

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