Fig 1: RCT increases FCN1_M-MDSC cells with elevated pro-inflammatory features. a UMAP visualization of the seven myeloid subclusters in all (left panel), pre-RCT (middle panel), and post-RCT (right panel) samples. b Heatmap showing the expression level of M1 and M2 signature genes in macrophage subclusters. c Bar plot showing the relative proportion of each myeloid cell subcluster in all myeloid cells in pre/post-RCT samples. d Box plots showing the fold change in the abundance of each myeloid cell subcluster compared between pre- and post-RCT samples. The colors of dots and squares represent patients and sample groups, respectively. The p values correspond to the paired Wilcoxon tests. e Representative images of IHC staining of FCN1 in paired pre-RCT and post-RCT FFPE tissues. Scale bar, 50 μm. f Box plot showing the fraction of FCN1+ M-MDSC cells in paired pre-RCT and post-RCT samples based on IHC results in e. The p value corresponds to the paired t test. g Box plot showing gene expression of FCN1 in pre-RCT and post-RCT bulk RNA-seq data. The p value corresponds to the paired t test. h Violin plots showing the expression of M1 and M2 signature genesets in myeloid cell subclusters in pre- and post-RCT groups (two-sided Wilcoxon test). i GO term analysis of M-MDSC cells in pre-RCT versus post-RCT samples. j GO term analysis of macrophages in pre-RCT versus post-RCT samples