Fig 1: L. pneumophila infection reduces total SLC35 protein levels and GalNAc supplementation promotes L. pneumophila growth in human macrophages. (A) Immunoblotting for SLC35A2 in U937 and HeLa cells infected with L. pneumophila. Cell lysates were probed with an anti-a-tubulin antibody as a loading control. (B) Densitometry results of 1 blot of U937 cells and 2 replicate HeLa cell blots, where a significant reduction in signal was observed in L. pneumophila-infected cells compared to control cells. * p < 0.05, one-way ANOVA, Tukey test. (C) Quantification of L. pneumophila fluorescence intensity after infection of U937 cells for 6 h, with the presence or absence of supplemental 20 mM GalNAc for the last 5 h of infection, prior to fixing and immunostaining with L. pneumophila antibodies. ** p < 0.01, n = 51 from three independent experiments, one-way ANOVA, Tukey test. Data represent mean ± S.E.M.
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