Fig 1: GTSE1 is highly expressed in OS and possibly correlates with unfavorable outcome. A expression profiling of GTSE1 in sarcoma in GEPIA; B, C expression of GTSE1 in OS tumor and para-tumorous tissues (n = 15) examined by RT-qPCR (B) and IHC staining (C), respectively (paired t test); D–E, expression of GTSE1 in OS cell lines (MNNG, MG-63, 143B, SaOS-2) and in normal hFOB 1.19 cells determined by RT-qPCR (D) and western blot analysis (E), respectively (one-way ANOVA vs. hFOB 1.19 cells); F, G overall survival (F) and disease-free survival (G) of patients with OS in the GEPIA database. Data were presented as the mean ± SD from three repetitions. *p < 0.05, **p < 0.01, ***p < 0.001
Fig 2: GTSE1 affects tumorigenesis of OS cells in nude mice following CDDP treatment. A Growth rate of xenograft tumors in each group of mice (two-way ANOVA); B weight of the xenograft tumors in each group of mice (one-way ANOVA); C, D GTSE1-positive (C) and Ki-67-positive (D) cells in xenograft tumors examined by IHC staining (one-way ANOVA); E portion of apoptotic cells in xenograft tumors determined by TUNEL assay (one-way ANOVA). Data were presented as the mean ± SD from three repetitions. *p < 0.05, **p < 0.01
Fig 3: GTSE1 enhances DNA damage repair-related biomarker in OS cells and reduces CDDP-induced cell apoptosis. A, B Protein levels of γH2AX and DNA-PKcs in MG-63 (A) and 143B (B) cells determined by western blot analysis (two-way ANOVA); C, D mRNA expression of DNA repair-related factors EXO1, PLK4, and RFC4 in MG-63 (C) and 143B (D) cells determined by RT-qPCR (two-way ANOVA). Data were presented as the mean ± SD from three repetitions. *p < 0.05, **p < 0.01
Fig 4: GTSE1 promotes S/G2 transition and DNA replication in OS cells. A mRNA expression of GTSE1 in MG-63 and 143B cells after sh-GTSE1 or oe-GTSE1 transfection, respectively, examined by RT-qPCR (unpaired t test); B, C cell cycle progression in MG-63 (B) and 143B (C) cells determined by flow cytometry (two-way ANOVA); D–E, protein levels of Cyclin D1, Cyclin E1, and PCNA in MG-63 (D) and 143B (E) cells determined by western blot analysis (two-way ANOVA); F, G DNA replication ability of MG-63 (F) and 143B (G) cells examined by EdU labeling assay (unpaired t test). Data were presented as the mean ± SD from three repetitions. *p < 0.05, **p < 0.01
Fig 5: GTSE1 is correlated with CDDP sensitivity of OS cells. A Proliferation of MG-63 and 143B cells after different doses of CDDP treatment determined by the CCK-8 method (two-way ANOVA); B IC50 value of CDDP in each group of cells (two-way ANOVA); C apoptosis rate in each group of cells after CDDP treatment at 3 μg/mL determined by flow cytometry (one-way ANOVA); D protein levels of apoptosis-related factors cleaved caspase 3, Bax, and Bcl-2 in each group of cells quantified by western blot analysis (two-way ANOVA). Data were presented as the mean ± SD from three repetitions. *p < 0.05, **p < 0.01
Supplier Page from Abcam for Anti-GTSE1 antibody