Fig 1: Mild, Transient Perturbations in Mitochondrial Function Are Associated with Increased Survival in Sepsis(A and B) CRIF1 and Cre recombinase protein expression (A) and Crif1 mRNA expression (B) in Crif1lox/lox or Crif1lox/− mice 7 days after injection of AAV8-TBG-expressing Cre recombinase (Ad.Cre) or GFP as a control (Ad.GFP).(C) Survival of Crif1lox/lox or Crif1lox/− mice, previously injected with AAV8-TBG-CRE, after infection with E. coli.(D) Bacterial loads in Crif1lox/− mouse blood 24 h after infection.(E) Survival of infected mice treated with phenformin at the time of infection.(F) Bacterial loads in blood 24 h after infection.(G) Enzymatic activity of the ETC complex I in liver at 12 h. ETC activity is expressed as % of PBS-treated control, normalized for CS activity.(H) HPLC-MS analysis of FAO metabolites in liver 8 h after infection and phenformin treatment. Each square represents one mouse.Data in (A) is from a single experiment. (B) represents mean ± SD of four mice per group assayed in triplicate. (C) represents pooled data from three independent experiments. (D) represents data from a single experiment. Bars indicate the mean. (E) represents pooled data from four independent experiments. (F) represents pooled data from two independent experiments. Bars indicate the mean. (G) represents mean ± SD of five mice per group from a single experiment. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. See also Figure S6.
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