Fig 1: Immuno-histochemical staining of the esophageal tissue of mice in control and model group at D35. (a,b) HE staining of the esophagus, which shows eosinophils in model group (b) outnumbered that in control group (a) in both mucosa and muscularis propria. * Indicates mucosa, ? indicates muscularis propria, black arrows indicate eosinophils. (c–h) Immuno-histochemical staining showed more MBP, ECP and EDN positive cells in the esophageal smooth muscle of mice in model group (d,f,h) compared with controls (c,e,g). Black arrows indicate MBP, ECP and EDN positive cells, respectively. (i–j) Immuno-histochemical staining for the localization of the primary antibody to SOX-10, which indicates ganglion cells in esophageal tissue of mice in model group (j) and control group (i). Black arrows indicate SOX-10 positive cells. Magnification ×400.