Fig 1: Expression of a cellular stress response protein. Immunofluorescence staining of the brain cortex in the peri-infarct region with the antibodies against heat shock protein 70 kDa (Hsp70) (green). Nuclei were counterstained with DAPI (blue). (A) Intact-mini pigs; (B) control mini-pigs 21 days after stroke modelling; (C) mini-pigs treated with the autologous genetically enriched leucoconcentrate 4 h after stroke modelling (TA group); (D) mini-pigs treated with the autologous genetically enriched leucoconcentrate 2 days before stroke modelling (TP group); (E) box plots demonstrate the mean (%) of the Hsp70-positive area in the intact and experimental groups. The scale of the images in (A) corresponds to that in (B–D). The asterisks in the schematic fragment of the cerebral cortex with the ischemic lesion indicate the areas used for the immunofluorescence analysis.