Fig 1: Preliminary experimental verification of the ACYP1 signature in LIHC. (A) Representative samples with low and high ACYP1 expression for IHC staining of ACYP1, CD4+ and CD8+ T cells in the ACYP1 low and high group were taken at 100x and 400x magnification. (B–D) Relative quantitative analysis of ACYP1 (B), CD4 (C) and CD8 (D) levels by IHC staining. (E) Survival analysis of the ACYP1 high group and the ACYP1 low group in 50 LIHC patients. (F) Subcutaneous growth of Hep1-6 tumors in the vector group and ACYP1 overexpression group (n=6). (G) Representative images of subcutaneous Hep1-6 tumors in the vector group and ACYP1 overexpression group (n=6). (H) The weight of subcutaneous Hep1-6 tumors in vector group and ACYP1 overexpression group (n=6). (I, J) The relative content of CD4+ T cells and CD8+ T cells in subcutaneous Hep1-6 tumors from vector mice and ACYP1 overexpressing mice (n=6). Positive cell percentages are shown. *P < 0.05, **P < 0.01, n.s. "no significance".
Fig 2: ACYP1 expression levels across cancers. Elevated or decreased levels of ACYP1 expression in multiple cancer types in Oncomine (A) and TIMER (B). *P < 0.05, **P < 0.01, ***P < 0.001.
Fig 3: Signal pathways involved in ACYP1. (A) Correlation between ACYP1 expression and KEGG pathways in LIHC analyzed via GSEA. (B) The top ten signaling pathways with significant positive and negative correlations with ACYP1 in LIHC. (C) Relationships between ACYP1 expression and KEGG pathways in LIHC performed via GSVA (NSE≥1.0, FDR<0.25).
Fig 4: Study flow-chart. I ACYP1 expression patterns explored via Oncomine, TIMER, and UALCAN; II Survival analysis of ACYP1 in PrognoScan, Kaplan—Meier Plotter, and GEPIA; III Signaling pathways and TME affected by ACYP1 via GSEA, GSVA, and TIMER; IV Experimental validation in human and mice tumor samples of LIHC.
Fig 5: The survival of patients with high and low expression of ACYP1 across cancers in the Kaplan–Meier Plotter database. OS and RFS of liver LIHC (A, B), BRCA (C, D), READ (E, F), HNSC (G, H), LUSC (I, J), LUAD (K, L), and SARC (M, N) patients. OS, overall survival; RFS, relapse-free survival.
Supplier Page from Abcam for Anti-ACYP1 antibody