Fig 1: PCAT6 upregulates the expression levels of PDIA6 via miR-143-3p. (A) The binding sites of miR-143-3p in 3'-untranslated region of PDIA6 were predicted by bioinformatics analysis. (B) Luciferase reporter assays were performed following co-transfection of the cells with WT-PDIA6 or MUT-PDIA6 and miR-143-3p mimics or NC mimics. (C-E) The (C) mRNA and (D and E) protein levels of PDIA6 were examined following transfection with siPCAT6 and anti-miR143. Data are presented as the mean ± standard deviation from three independent experiments. *P<0.05. miR, microRNA; si, small interfering RNA; NC, negative control; PCAT6, prostate cancer-associated transcript 6. WT, wild-type; MUT, mutant; PDIA6, protein disulfide isomerase A6.
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