Fig 1: The protein staining of the endovascular extravillous trophoblasts of the placental bed for the 22 markers evaluated by immunohistochemistry. The endovascular extravillous trophoblasts are found in the tunica media of the vessel wall of the spiral arteries. For CK7, there is shown a double staining of CK7-positive endovascular extravillous trophoblasts in brown and CD34-positive endothelial cells in pink. SA: spiral artery, vasEVT: endovascular extravillous trophoblasts. Proteins significantly expressed in vasEVT: AQPEP, CK7, CNR1, FERMT2, FLT1, GPX8, PRTG, UCHL1, and UPK1B.
Fig 2: The protein staining of the interstitial extravillous trophoblasts of the basal plate and basal decidua for the 22 markers evaluated by immunohistochemistry. DSC: decidual stromal cells, iEVT: interstitial extravillous trophoblasts. Proteins significantly expressed in iEVT: AQPEP, CK7, CNR1, FERMT2, FLT1, GPX8, PRTG, PVRL4, THY1, and UCHL1.
Fig 3: The protein staining of the fetal membrane cells for the 22 markers evaluated by immunohistochemistry. AEC: amnion epithelial cells, AMSC: amnion mesenchymal stromal cells, CMSC: chorion mesenchymal stromal cells, CTC: chorion trophoblast cells, DSC: decidual stromal cells. Proteins significantly expressed in AEC: AHNAK2, CK5, CK7, CK17, CNR1, DPYSL3, EMP1, FERMT2, FLT1, GPX8, MUC16, PRLR, PVRL4, RXFP1, UCHL1, UPK1B, and VIM; proteins significantly expressed in AMSC: AHNAK2, DPYSL3, EMP1, FLT1, GPX8, PRLR, RXFP1, UCHL1, and VIM; proteins significantly expressed in CMSC: AHNAK2, DPYSL3, EMP1, FLT1, GPX8, PDLIM4, PRLR, THY1, UCHL1, and VIM; proteins significantly expressed in CTC: CK7, CNR1, FLT1, GPX8, PRTG, PVRL4, UCHL1, and UPK1B; proteins significantly expressed in DSC: AHNAK2, DPYSL3, FERMT2, FLT1, GPX8, PDLIM4, PRTG, THY1, UCHL1, and VIM.
Fig 4: The protein staining of the peripheral villi of the placenta for the 22 markers evaluated by immunohistochemistry. VT: villous trophoblasts (primarily the syncytiotrophoblast), VSC: villous stromal cells, FBV: fetal blood vessel. Proteins significantly expressed in VT: CK7, CNR1, FERMT2, FLT1, PRTG, PVRL4, RXFP1, and UCHL1.
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