Fig 1: Flow cytometric analyses of moving HepELs in a KikGR mouse lungs A–ERepresentative flow cytometric analysis in lungs 72 h after liver exposure to violet light. Cells obtained from mouse with no light exposure gave KikGR red versus KikGR green dot plot (A). Those with light exposure (B, D) exhibited photoconverted cells in a red polygonal region. Cells in the gated region were further analysed by using CD4, CD8, CD45, CD11c, CD11b and B220 antibodies (C, E). Percent is shown for total cells in (A, B and D) and for gated cells in (C and E). conCM (control) and TCM were injected (3 times, every 2 days). Comparison of isotype control-Ab and specific-Ab are also shown (Appendix Figs S3 and S4).
Fig 2: Activation of TGF-ß signaling reduces the inflammatory infiltration and demyelination in the optic nerve of EAE mice. (A) The body weight of control-treated YAPf/f (n = 10), control-treated YAPGFAP-CKO mice (n = 7), SRI-011381-treated YAPf/f (n = 9) and SRI-011381-treated YAPGFAP-CKO mice (n = 8) from 0 to 22 dpi during the process of EAE modeling. (B) The EAE score of control-treated YAPf/f mice (n = 10), control-treated YAPGFAP-CKO mice (n = 7), SRI-011381-treated YAPf/f mice (n = 9) and SRI-011381-treated YAPGFAP-CKO mice (n = 8) 0 to 22 dpi during the process of EAE modeling. (C) LFB staining in the optic nerve of control-treated YAPf/f EAE and YAPGFAP-CKO EAE mice, SRI-011381-treated YAPf/f EAE and YAPGFAP-CKO EAE mice. (D) Quantitative analysis of the demyelination score as shown in (C) (n = 7 per group). (E, G, I, K) Immunostaining of MBP (green) (E), Iba1 (green) (G), GFAP (green) (I) or CD45 (green) (K) in the optic nerve of control-treated YAPf/f EAE and YAPGFAP-CKO EAE mice, SRI-011381-treated YAPf/f EAE and YAPGFAP-CKO EAE mice. (F, J) Quantitative analysis of the relative intensity of MBP (F) or GFAP (J) as shown in (E, I) (n = 6 per group, normalized to control). (H, L) Quantitative analysis of the density of Iba1+ cells (H) or CD45+ cells (L) as shown in (G, K) (n = 7 per group). Images of selected regions (white squares) were shown at higher magnification. Data were mean ± SEM, two-way ANOVA with Bonferroni's post-tests, compared with control group, *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 20 µm.
Fig 3: Increased numbers of CD45-positive cells in BDLR mice. (a) Quantification of the percent area of CK19-positive stain was significantly increased in BDL mice versus sham controls. There was no difference in percent of positive Sirius Red stain between groups. (b) Cell quantification demonstrated increased numbers of CD45-positive cells in BDLR mice compared to BDL. Ly6g-positive cells were increased only in BDLR mice versus their sham controls. *p < .05
Fig 4: CD45 Immunohistochemistry of gonadal adipose tissues. (a) Representative CD45 immunohistochemistry staining of gonadal adipose tissues from six month-old female wildtype (WT), aromatase knockout (KO) and 2.5µg/day 17ß-estradiol-treated KO (KOE) mice; (b) quantitated CD45-positive cells. Data are presented as mean ± SD (n = 5/group). *p< 0.05, versus age-matched WT samples.
Fig 5: Tissue from a representative first miscarriage, 9th week of gestation.Paraffin serial sections. A subpopulation of Hofbauer cells residing in the villous stroma, identified by CD68 (panel b,d,f), are positive for CD100 (panel a, red asterisks), CD72 (panel c, red asterisk) and CD45 (panel e, red asterisks). a,b,c,d,e,f,: Bar = 60 µm.
Supplier Page from Abcam for Anti-CD45 antibody