Fig 1: METTL3 and FTO modulate ATM expression. (A) BJAB cells were treated with 10 µM 3-DAA (control group were treated with equal amount of PBS) for 2 h, cellular RNA was extracted at 24 h and mRNA expression of ATM was assayed by RT-qPCR (n=3). Western blot assay was used to detect ATM protein expression. (B) Western blot assay was used to detect ATM and METTL3 protein expression levels in sh-NC or sh-METTL3 cells. (C) RT-qPCR was used to detect ATM and METTL3 mRNA expression levels in sh-NC or sh-METTL3 BJAB cells. (D) BJAB cells (sh-NC and sh-METTL3) were treated with Act-D (5 µg/ml) for indicated times, then, RT-qPCR assay was performed to detect ATM mRNA expressions. Mean ± SD of three independent experiments. Two-tailed unpaired t-test. (E) SGC7901 cells were transfected with indicated siRNAs for 24 h. ATM mRNA expression was detected by RT-qPCR. (F) SGC7901 cells (si-NC and si-FTO) were treated with Act-D (5 µg/ml) for indicated times, then, RT-qPCR assay was performed to detect ATM mRNA expressions. (G) SGC7901 cells were treated with indicated siRNAs for 48 h, Western blot was used to detect the indicated proteins. *p < 0.05, **p < 0.01, ***p < 0.001. ns, no significant difference.