Anti-SMARCA2 / BRM antibody ab15597 from Abcam

Product Specs
ItemAnti-SMARCA2 / BRM antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab15597
Antibody TypePrimary
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
Quantity100 UG
ClonalityPolyclonal
HostRabbit
IsotypeIgG
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameSMARCA2
ReactivityHuman, Mouse
TargetSMARCA2 / BRM

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) SWI/SNF chromatin remodeler complex within the reward pathway is required for behavioral adaptations to stressNature CommunicationsDecember 1, 2022Abdallah Zayed et al.Santa Cruz, sc-17796, clone G-7, Lot H1314) and an anti-BRM (rabbit polyclonal 1:500, Abcam, Catalog ab15597, Lot GR49552-3). The following day, the supernatants were washed three times with 1 ml PBS/Tween and

(2) BCL7A-containing SWI/SNF/BAF complexes modulate mitochondrial bioenergetics during neural progenitor differentiationThe EMBO journalDecember 1, 2022Wischhof L, Lee HM, Tutas J, Overkott C, Tedt E, Stork M, Peitz M, Brüstle O, Ulas T, Händler K, et al.(ab110641, Abcam), mouse anti‐BRG1 (MA5‐31550, Invitrogen), rabbit anti‐BRM (11966, Cell Signaling and ab15597, Abcam), rabbit anti‐α 1 Fetoprotein (ab284388, Abcam), rabbit anti‐cFos (226003, Synaptic Systems),

(3) SMARCA4 biology in alveolar rhabdomyosarcomaOncogeneMarch 10, 2022Narendra Bharathy, Megan M. Cleary, Jin-Ah Kim, Kiyo Nagamori, Kenneth A. Crawford, Eric Wang, Debarya Saha, Teagan P. Settelmeyer, Reshma Purohit, Damianos Skopelitis, et al.analysis with anti-SMARCA4/Brg-1 (sc-17796, Santa Cruz Biotechnology, Dallas, TX, USA), anti-SMARCA2 (ab15597, Abcam, San Francisco, CA, USA), anti-PBRM1 (A301–59A, Bethyl Laboratories, Montgomery, TX, USA), anti-β-actin

(4) Smarca4 Inactivation Promotes Lineage-Specific Transformation and Early Metastatic Features in the LungCancer DiscoveryFebruary 1, 2022Carla P. Concepcion et al.antibodies were used: anti-SMARCA4 (Abcam Cat# ab110641, RRID:AB_10861578), anti-SMARCA2 (Abcam Cat# ab15597, RRID:AB_443214), anti-ARID1A (Abcam, ab217154 and Cell Signaling Technology Cat# 12354, RRID:AB_2637010),

(5) Targeting histone acetylation dynamics and oncogenic transcription by catalytic P300/CBP inhibitionMolecular CellMay 20, 2021Simon J. Hogg et al.#5848 "Anti-BRG1, rabbit monoclonal",Abcam,Cat#ab110641 "Anti-SMARCA2/BRM, rabbit polyclonal",Abcam,Cat#ab15597 "Anti-RNA Polymerase II, mouse monoclonal",MilliporeSigma,Cat#05–623 "Anti-NCOR1, rabbit polyclonal",Diagenode,Cat#C15410341 "Anti-H3K27me3,

(6) Loss of BAF Complex in Developing Cortex Perturbs Radial Neuronal Migration in a WNT Signaling-Dependent MannerFrontiers in molecular neuroscienceJanuary 1, 2021Sokpor G, Kerimoglu C, Nguyen H, Pham L, Rosenbusch J, Wagener R, Nguyen HP, Fischer A, Staiger JF, Tuoc T.Cat. 611728; BD), BAF155 rabbit pAb (1:20; Cat. sc-10756; Santa Cruz), BRM rabbit pAb (1:200; Cat. ab15597; Abcam), BRG1 rabbit pAb (1:120; Cat. sc-10768X; Santa Cruz), BAF155 mouse mAb (1:100; Cat. sc-48350X;

(7) Loss of SWI/SNF Chromatin Remodeling Alters NRF2 Signaling in Non-Small Cell Lung CarcinomaMolecular cancer research : MCRDecember 1, 2020Song S, Nguyen V, Schrank T, Mulvaney K, Walter V, Wei D, Orvis T, Desai N, Zhang J, Hayes DN, et al.Santa Cruz Biotechnology, Dallas, TX), BRG1 (G7, sc17796, Santa Cruz Biotechnology, Dallas TX), BRM (ab15597, Abcam, Cambridge, England) and β-ACTIN (A2066, Sigma, St. Louis, MO). Quantitative RT-PCR Total RNA

(8) Functional characterization of SMARCA4 variants identified by targeted exome-sequencing of 131,668 cancer patientsNature communicationsNovember 3, 2020Fernando TM, Piskol R, Bainer R, Sokol ES, Trabucco SE, Zhang Q, Trinh H, Maund S, Kschonsak M, Chaudhuri S, et al.300-500 bp. ChIPs were performed with 30 μg of precleared chromatin and 5 μl of anti-SMARCA2 (Abcam, ab15597) or 10 μL anti-SMARCA4 antibody (Abcam, ab110641). Complexes were washed, eluted from the beads with

(9) BRG1 Is Dispensable for Sertoli Cell Development and Functions in MiceInternational Journal of Molecular SciencesJune 19, 2020Shuai Wang, Pengxiang Wang, Dongli Liang, Yuan Wang(ab212951), GFP (ab183734), TRA98 (ab82527), SYCP3 (ab15093), SYCP3 (ab97672), TNP1 (ab73135), and BRM (ab15597) were purchased from Abcam (Cambridge, MA, USA). PLZF (sc-28319) was purchased from Santa Cruz Biotechnology

(10) Small Cell Carcinoma of the Ovary, Hypercalcemic Type (SCCOHT) beyond SMARCA4 Mutations: A Comprehensive Genomic AnalysisCellsJune 19, 2020Aurélie Auguste et al.assessed by immunohistochemistry (IHC) using the anti-BRG1 (Santa Cruz, sc-10768) and anti-BRM (Abcam, ab15597) antibodies at dilutions of 1/200 and 1/50, respectively. After paraffin removal and hydration, slides

(11) BRM-SWI/SNF chromatin remodeling complex enables functional telomeres by promoting co-expression of TRF2 and TRF1PLoS geneticsJune 1, 2020Wu S, Ge Y, Li X, Yang Y, Zhou H, Lin K, Zhang Z, Zhao Y.Cell Signaling Technology), TPP1 (1:1000 dilution, 14667, Cell Signaling Technology), BRM (1:2000 dilution, ab15597, Abcam), BRG1 (1:2000 dilution, 21634-1-AP, Proteintech) and Flag (1:5000 dilution, F1804, Sigma). β-actin

(12) Clinical utility of SMARCA4 testing by immunohistochemistry in rare ovarian tumoursBritish Journal of CancerFebruary 18, 2020Catherine Genestie et al.performed using the rabbit polyclonal antibody anti-BRG-1 (Santa Cruz, sc-10768) and anti-BRM (Abcam, ab15597), respectively, at a dilution of 1/200 and 1/50. After paraffin removal and hydration, the slides were

(13) Hypermethylation of BRM promoter plays oncogenic roles in development of clear cell renal cell carcinomaJournal of CancerAugust 28, 2019Ru Fang et al.USA). The expression of BRM was assessed by immunoblotting using an antibody purchased from Abcam (ab15597, Abcam, Cambridge, MA, USA) and normalized to that of GAPDH. Analysis of apoptosis by flow cytometry After

(14) The SWI/SNF complex is a mechanoregulated inhibitor of YAP and TAZNatureNovember 1, 2018Chang L, Azzolin L, Di Biagio D, Zanconato F, Battilana G, Lucon Xiccato R, Aragona M, Giulitti S, Panciera T, Gandin A, et al.and anti-Actin (A5316) were from Sigma; anti-YAP (ab52771), anti-Histone H3 (ab1791) and anti-BRM (ab15597) were from Abcam; anti-GAPDH (MAB347) and anti-ARID1A (04-080) monoclonal antibody were from Millipore.

(15) SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3′ end processingNucleic Acids ResearchSeptember 19, 2018Simei Yu, Antonio Jordán-Pla, Antoni Gañez-Zapater, Shruti Jain, Anna Rolicka, Ann-Kristin Östlund Farrants, Neus Visaet al. (29), respectively. We also used the following commercial antibodies from Abcam: anti-hBrm (ab15597), anti-RNAPII CTD (ab5408), anti-tubulin (ab7291), anti-hCPSF1 (ab81552), anti-hCPSF2 (ab126760), anti-HA

(16) Partial Inactivation of the Chromatin Remodelers SMARCA2 and SMARCA4 in Virus-Infected Cells by Caspase-Mediated CleavageJournal of VirologyAugust 15, 2018Alexandra H. Dudek, Florian Pfaff, Hardin Bolte, Collins Waguia Kontchou, Martin SchwemmleSigma-Aldrich]; anti-NDV-serum, kindly provided by Georg Kochs [1:1,000]; anti-SMARCA2/BRM N terminus [ab15597; 1:1,000; Abcam]; anti-SMARCA2/BRM C terminus [ab12165; 1:10,00; Abcam]; anti-SMARCA4/BRG1 N terminus

(17) Tumor suppression via inhibition of SWI/SNF complex-dependent NF-κB activationScientific ReportsDecember 1, 2017Kazuyoshi Kobayashi, Hiroaki Hiramatsu, Shinya Nakamura, Kyousuke Kobayashi, Takeshi Haraguchi, Hideo Ibaincubating the membranes overnight at 4 °C with primary antibodies against the following proteins: Brm (ab15597; Abcam), BRG1 (sc-10768; Santa Cruz), Halo tag (G928A; Promega), HA tag (#3724; Cell Signaling), and

(18) Genome-wide analysis reveals positional-nucleosome-oriented binding pattern of pioneer factor FOXA1Nucleic Acids ResearchSeptember 19, 2016Zhenqing Ye, Zhong Chen, Benjamin Sunkel, Seth Frietze, Tim H.-M. Huang, Qianben Wang, Victor X. JinSanta Cruz Biotechnology (Santa Cruz, CA), CHD4 (ab72418), SNF2H (ab72499), SMARCA1 (ab37003), SMARCA2 (ab15597) from Abcam (Cambridge, UK). Data access The ChIP-seq data from this study has been submitted to the

(19) Chromatin Regulators as a Guide for Cancer Treatment ChoiceMolecular Cancer TherapeuticsJuly 1, 2016Zachary A. Gurard-Levin et al.reader (Perkin Elmer). We used commercial antibodies: SMARCA4 (Santa Cruz sc-17796), SMARCA2 (Abcam ab15597), ARID1B (Abcam ab57461, gamma-tubulin (Sigma T5326), ARID1A (Santa Cruz sc-32761), ARID2 (Santa Cruz

(20) Brg1 plays an essential role in development and homeostasis of the duodenum through regulation of Notch signalingDevelopmentJune 24, 2016Yutaka Takada, Akihisa Fukuda, Tsutomu Chiba, Hiroshi Senosc-25392, Santa Cruz Biotechnology), rabbit antiNumb (1:100; ab14140, Abcam), and rabbit anti-Brm (1:400; ab15597, Abcam). Antigen retrieval for all primary antibodies was achieved by boiling in 10 mM citrate buffer

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8/23/2017Brm Staining In Pancreatic Cryosection Jason SpaethOur focus is on the influence Brm, an ATPase subunit of the Swi/Snf chromatin remodeling complex, has in pancreatic islets. Staining mouse pancreas cryosections with the rabbit polyclonal antibody ... Read Review

Anti-SMARCA2 / BRM antibody from Abcam

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Anti-SMARCA2 / BRM antibody from Abcam

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