Anti-6X His tag® antibody [HIS.H8] ab18184 from Abcam

Product Specs
ItemAnti-6X His tag® antibody [HIS.H8]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab18184
Antibody TypePrimary
ClonalityMonoclonal
HostMouse, Nude mouse
ApplicationsDot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
CloneHIS.H8
IsotypeIgG2b
Conjugate/TagUnconjugated
Concentration1 mg/ml
ReactivitySpecies independent
Target6X His tag®
Quantity100 UG

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Dapoxetine, a Selective Serotonin Reuptake Inhibitor, Suppresses Zika Virus Infection In VitroMoleculesDecember 17, 2023Bingzhi Zhang, Jianchen Yu, Ge Zhu, Yun Huang, Kexin Zhang, Xuhan Xiao, Wenxuan He, Jie Yuan, Xiaoxia GaoInc., Irvine, CA, USA), anti-ZIKV NS5 (GTX133312, 1:1000, GeneTex Inc., Irvine, CA, USA), 6x His-tag (ab18184, 1:1000. Abcam, Shanghai, China) and anti-GAPDH (60004-1-IG, 1:7000, Proteintech, Rosemont, IL, USA).

(2) A bead-based multiplex assay covering all coronaviruses pathogenic for humans for sensitive and specific surveillance of SARS-CoV-2 humoral immunitySci RepDecember 9, 2023Daniel Stern, Tanja C. Meyer, Fridolin Treindl, Hans Werner Mages, Maren Krüger, Martin Skiba, Jan Philipp Krüger, Christian M. Zobel, Maximilian Schreiner, Marica Grossegesse, et al.determined by His-tag specific ELISA using a mouse anti-His-tag antibody (Abcam, Berlin, Germany, #ab18184) and a goat anti-mouse IgG Fc antibody conjugated to alkaline phosphatase (Southern Biotech, Biozol,

(3) Evaluation of variable new antigen receptors (vNARs) as a novel cathepsin S (CTSS) targeting strategyFront PharmacolDecember 5, 2023P. Smyth, L. Ferguson, J. F. Burrows, R. E. Burden, S. R. Tracey, Ú. M. Herron, M. Kovaleva, R. Williams, A. J. Porter, D. B. Longley, et al.used as follows: goat anti-human-CTSS (RnD Systems, AF-1183) (1:2000) and mouse anti-6X-his (Abcam, ab18184) (1:1000) with subsequent incubation with secondary antibodies donkey anti-goat-HRP (Santa Cruz, sc-2056)

(4) IL-6/JAK2-dependent G6PD phosphorylation promotes nucleotide synthesis and supports tumor growthMolecular MetabolismDecember 1, 2023Xuemei Qiu, Hongping Ye, Xiaofei Li, Dan Li, Lu Jiang, Rui Liu, Zhe Zhao, Dan Hepurchased from Proteintech (HZ-1019). Antibodies recognizing Tubulin (ab7291), Flag (ab205606), His (ab18184) and G6PD (ab231828) were purchased from Abcam. [γ-32P]-ATP was purchased from PerkinElmer (BLU002Z001MC).

(5) Development of a Novel ATP Bioluminescence Assay Based on Engineered Probiotic Saccharomyces boulardii Expressing Firefly LuciferaseJournal of Microbiology and BiotechnologyNovember 28, 2023Ji Sun Park, Young-Woo Kim, Hyungdong Kim, Sun-Ki Kim, Kyeongsoon Parkimmunoblot assays, His6-fusion proteins were detected using an anti-polyhistidine antibody (Abcam, ab18184) according to the manufacturer’s instructions. In Vitro Detection of ATP To evaluate whether the SKSC175

(6) Recombinant cellular model system for human muscle-type nicotinic acetylcholine receptor α12β1δεCell Stress and ChaperonesNovember 25, 2023Sabrina Brockmöller, Thomas Seeger, Franz Worek, Simone Rothmillerblocking buffer for 2.5 h at 180 rpm at room temperature (Abcam antibodies for native detection: His-tag ab18184, HA-tag ab49969, myc-tag ab32, falg-tag ab236777, strep-tag-II ab180957, rho1D4-tag ab5417, RFP ab185921,

(7) The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylationVirusesNovember 24, 2023Congcong Huang, Yiping Yin, Pan Pan, Yanping Huang, Siwei Chen, Junkai Chen, Ju Wang, Guoqing Xu, Xuan Tao, Xiao Xiao, et al.utilizing either an anti-Flag antibody (Sigma, Cat No. F1084) or an anti-His6 antibody (Abcam, Cat No. ab18184). Pierce™ Protein A/G Magnetic Beads (Thermo Scientific, Cat No. 88802) were employed for the immunoprecipitation.

(8) A 5+1 assemble-to-activate mechanism of the Lon proteolytic machineNature CommunicationsNovember 13, 2023Shanshan Li, Kan-Yen Hsieh, Chiao-I Kuo, Tzu-Chi Lin, Szu-Hui Lee, Yi-Ru Chen, Chun-Hsiung Wang, Meng-Ru Ho, See-Yeun Ting, Kaiming Zhang, et al.Western blot analysis using a 6x-His tag monoclonal antibody at a 1:10,000 dilution (clone HIS.H8, Abcam ab18184), and further evaluated by Ni-NTA chromatography with production yields of 19.2 and 21.1 mg/L of culture,

(9) SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitinationPLOS PathogensNovember 13, 2023Xiaolin Zhang, Ziwei Yang, Ting Pan, Qinqin Sun, Qingyang Chen, Pei-Hui Wang, Xiaojuan Li, Ersheng Kuanganti-phospho-NF-κB p65 (#3033) and anti-NF-κB p65 (#8242), purchased from Cell Signaling Technology; anti-His (ab18184), purchased from Abcam; anti-TRIM4 (#AP13289a), purchased from abcepta; anti-MDA5/IFIH1 (66770-1-Ig),

(10) Efficient 3′-pairing renders microRNA targeting less sensitive to mRNA seed accessibilityNucleic Acids ResearchNovember 10, 2023David M Kosek, Elnaz Banijamali, Walter Becker, Katja Petzold, Emma R Anderssonfinal concentration of 2%. AGO2 overexpression was verified by Western blot (Abcam, anti-6X His-tag AB [ab18184]). Sf9 cells grown in Sf-900 II SFM media (Thermo Fisher) were transducted with P2-AGO2 baculovirus

(11) The heat response regulators HSFA1s promote Arabidopsis thermomorphogenesis via stabilizing PIF4 during the dayScience AdvancesNovember 3, 2023Wenrong Tan, Junhua Chen, Xiaolan Yue, Shuli Chai, Wei Liu, Chenglin Li, Feng Yang, Yongfeng Gao, Lucas Gutiérrez Rodríguez, Víctor Resco de Dios, et al.catalog no. cw0264A), anti-MBP (New England Biolabs, catalog no. E8023), and anti-His (Abcam, catalog no. ab18184) were used for detected Myc-PIF4, HSFA1d-GFP, PIF4, ACTIN2, MBP-HSFA1d, and His-PIF4, respectively.

(12) Outer membrane vesicles from a mosquito commensal mediate targeted killing of Plasmodium parasites via the phosphatidylcholine scavenging pathwayNat CommunAugust 24, 2023Han Gao, Yongmao Jiang, Lihua Wang, Guandong Wang, Wenqian Hu, Ling Dong, Sibao Wanganti-HA antibody (CST, cat#3724 S) at a dilution of 1:1000; mouse monoclonal Anti-His tag antibody (ab18184, Abcam) at a dilution of 1:1000; anti-CD63 rabbit monoclonal antibody (A22343, ABclonal) at a dilution

(13) A novel approach to enhance the performance of kallikrein 6 enzyme using Pichia pastoris GS115 as a hostResearch in Pharmaceutical SciencesAugust 20, 2023Fatemeh Mahmoodi, Hamid Bakherad, Navid Mogharrab, Mohammad RabbaniBradford kit and mouse anti-His tag specific antibody were provided from Sinagen (Iran) and Abcam (ab18184) (USA), respectively. Preparation of engineered pro-KLK6 and cloning of recombinant KLK6 in expression

(14) HSPA8 acts as an amyloidase to suppress necroptosis by inhibiting and reversing functional amyloid formationCell ResearchAugust 14, 2023Erpeng Wu, Wenyan He, Chenlu Wu, Zhangcheng Chen, Shijie Zhou, Xialian Wu, Zhiheng Hu, Kelong Jia, Jiasong Pan, Limin Wang, et al.Prosci (anti-mouse RIP3, Cat# 2283) and Abcam (anti-LAMP2A, ab125068; anti-V5-HRP, ab1325; anti-6×His, ab18184; anti-human p-MLKL, ab187091; anti-mouse p-MLKL, ab196436). Anti-human MLKL, anti-mouse MLKL and anti-human

(15) Ubiquitination of acetyltransferase Gcn5 contributes to fungal virulence in Fusarium graminearummBioJuly 28, 2023Ahai Chen, Yifan Zhou, Yiyi Ren, Chao Liu, Xingmin Han, Jing Wang, Zhonghua Ma, Yun Chentransfer, anti-GFP (ab32146; Abcam, Cambridge, UK), anti-mCherry (ab125096, Abcam), anti-His antibody (ab18184, Abcam), anti-H3 (no. 61475; Active Motif, La Hulpe, Belgium), anti-H3K18ac (no. 39756, Active Motif),

(16) The Piezo channel is central to the mechano-sensitive channel complex in the mammalian inner earResearch SquareJuly 13, 2023Jeong-Han Lee, Cristina Perez-Flores, Seojin Park, Hyo Jeong Kim, Yingying Chen, Mincheol Kang, Jennifer Kersigo, Jinsil Choi, Phung N. Thai, Ryan Woltz, et al.(CS-Mm07673-M02–01J). Antibodies were as follows: anti-HA (Abcam, cat no. ab9110, ab18181), anti-His (Abcam, cat no. ab18184, ab9108), anti-Myc (Abcam, cat no. ab32, ab9106), anti-mCherry (Abcam, cat no. ab183628, ab125096) and

(17) Forkhead box protein D2 suppresses colorectal cancer by reprogramming enhancer interactionsNucleic Acids ResearchJuly 7, 2023Hyo-Min Kim, Byunghee Kang, Sohyun Park, Hyorim Park, Chan Johng Kim, Hyeonji Lee, Mijoung Yoo, Mi-Na Kweon, Sin-Hyeog Im, Tae Il Kim, et al.ab8895), H3K27ac (Abcam, ab4729), MLL4/KMT2D (Santa Cruz, sc-293217), Anti-6× His tag® [HIS.H8] (Abcam, ab18184) or monoclonal ANTI-FLAG® M2 (Sigma-Aldrich, F1804) overnight at 4 °C with rotation. The pulled chromatin

(18) Spt6 directly interacts with Cdc73 and is required for Paf1 complex occupancy at active genes in Saccharomyces cerevisiaeNucleic Acids ResearchJune 9, 2023Mitchell A Ellison, Sanchirmaa Namjilsuren, Margaret K Shirra, Matthew S Blacksmith, Rachel A Schusteff, Eleanor M Kerr, Fei Fang, Yufei Xiang, Yi Shi, Karen M ArndtH3K4me3 (Active Motif 39159, 1:2000), HA tag (Roche 11666606001, 1:1000 or 1:3000), His tag (Abcam ab18184, 1:1000), HSV tag (Sigma-Aldrich H6030, 1:1000 or 1:2000), Rpb1-Core (Y80 Santa Cruz, SC-25758, 1:1000),

(19) A superior extracellular matrix binding motif to enhance the regenerative activity and safety of therapeutic proteinsnpj Regenerative MedicineMay 22, 2023Yasmin K. Alshoubaki, Yen-Zhen Lu, Julien M. D. Legrand, Rezvan Karami, Mathilde Fossat, Ekaterina Salimova, Ziad Julier, Mikaël M. MartinoConcentrations of proteins were determined by ELISA using an anti-histidine tag capture antibody (Abcam, ab18184) and a detection antibody from PDGF-BB or IL-1Ra/IL-1F3 DuoSet ELISA kit (R&D Systems). The day 0 samples

(20) Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndromeCellMay 11, 2023Healy MD, McNally KE, Butkovič R, Chilton M, Kato K, Sacharz J, McConville C, Moody ERR, Shaw S, Planelles-Herrero VJ, et al.GTX121488 HA-tag,Insight Biotechnology,Cat# A02040 Strep-tag,GeneTex,Cat# GTX128061 6x His-tag,Abcam,Cat# Ab18184 Myc-tag,GeneTex,Cat# GTX115046 FLAG tag,Sigma,Cat# F3165 SNAP-tag,GeneTex,Cat# GTX54523 Alexa Fluor

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1/30/2023Great His Tag Antibody for Western Blot Stephanie BaringerUsed to determine the efficiency of His-tag protein transfection. It gives multiple bands, but there are other proteins with his tags. Used in HEK 293 cells.

Anti-6X His tag® antibody [HIS.H8] from Abcam

Abcam

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Anti-6X His tag® antibody [HIS.H8] from Abcam

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