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Anti-HA tag antibody [HA.C5]
Anti-HA tag antibody [HA.C5] from Abcam
Be the first to write a review!
Citations:
(228)
Supplier Page
Supplier Page from
Abcam for
Anti-HA tag antibody [HA.C5]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-HA tag antibody [HA.C5]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab18181
Antibody Type
Primary
Host
Mouse, Nude mouse
Clone
HA.C5
Quantity
100 UG
Clonality
Monoclonal
Isotype
IgG3
Applications
Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Gene Name
HA
Reactivity
Species independent
Target
HA tag
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (228)
(1)
TRIM25 dictates selective miRNA loading into extracellular vesicles during inflammation
Sci Rep
December 22, 2023
Kayla E. King, Priyanka Ghosh, Ann L. Wozniak
Protease inhibitor cocktail
(P8340; Sigma-Aldrich) was used at
1:100
dilution. Antibodies used included HA (
Abcam
,
ab18181
),
TRIM25
(
Novus
,
NBP2-61814
), Lys63-specific ubiquitin (
05-1308
), TSG101 (EPR7130(B)), and Flag M2 (
Sigma
,
(2)
TLR7 neo-functionalizes to sense dsRNA and trigger antiviral and antibacterial immunity in non-tetrapod vertebrates
iScience
December 15, 2023
Rui Jiang, Wentao Zhu, Zhiwei Liao, Chunrong Yang, Jianguo Su
monoclonal Ab,Abcam,
Abcam
,Cat#
ab291
; RRID: AB_449092 Mouse anti-HA monoclonal Ab,Abcam,
Abcam
,Cat#
ab18181
; RRID: AB_444303 Mouse anti-Flag monoclonal Ab,Creative Biomart,
Creative Biomart
,Cat#
CAB551
; RRID:
(3)
Demonstrating the importance of porcine reproductive and respiratory syndrome virus papain-like protease 2 deubiquitinating activity in viral replication by structure-guided mutagenesis
PLoS Pathog
December 14, 2023
Ben A. Bailey-Elkin, Robert C. M. Knaap, Anuradha De Silva, Ilse M. Boekhoud, Sandra Mous, Niek van Vught, Mazdak Khajehpour, Erwin van den Born, Marjolein Kikkert, Brian L. Mark
Sigma-Aldrich
), rabbit-α-
GFP
[9],
mouse
-α-V5 (
37–7500
,
Invitrogen
),
mouse
-α-myc (9E10, Roche),
mouse
-α-HA (
ab18181
,
Abcam
), and
mouse
-α-FLAG (
F1804
,
Sigma-Aldrich
). As secondary antibodies,
goat
-α-
mouse
-HRP (P0447,
(4)
AtMYBS1 negatively regulates heat tolerance by directly repressing the expression of MAX1 required for strigolactone biosynthesis in Arabidopsis
Plant Communications
November 13, 2023
Xiang Li, Jianhua Lu, Xuling Zhu, Yanqi Dong, Yanli Liu, Shanshan Chu, Erhui Xiong, Xu Zheng, Yongqing Jiao
After blocking, the
membrane
was sequentially incubated with primary antibody (anti-HA,
Abcam
, UK, #
ab18181
) and secondary antibody (
mouse
HRP,
Abcam
, #
ab131368
) at room temperature for 2 h. The chemiluminescent
(5)
Interferon-stimulated gene TDRD7 interacts with AMPK and inhibits its activation to suppress viral replication and pathogenesis
mBio
October 31, 2023
Sukanya Chakravarty, Gayatri Subramanian, Sonam Popli, Manoj Veleeparambil, Shumin Fan, Ritu Chakravarti, Saurabh Chattopadhyay
(
Sigma-Aldrich
#
A5441
), anti-V5 (
Thermo Fisher Scientific
#
R960-25
and
Cell Signaling
#
13202S
), anti-HA (
Abcam
#
ab18181
and
CST
#
3724
), anti-His (
Qiagen
#
34660
), anti-Flag (
CST
#
2368S
), anti-ICP0 (
Abcam
#
ab6513
and
Santa Cruz
(6)
The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation
Nucleic Acids Research
October 27, 2023
Yilin Wang, Patrik Risteski, Yang Yang, Huan Chen, Gaith Droby, Andrea Walens, Deepika Jayaprakash, Melissa Troester, Laura Herring, Jonathan Chernoff,
et al.
antibody—ChIP Grade (
Abcam
,
ab9110
), Mouse Anti-HA tag Monoclonal Antibody, Unconjugated,
Clone
HA.C5 (
Abcam
,
ab18181
), c-Myc antibody [9E10] - ChIP Grade (
Abcam
,
ab32
),
Nuclear Pore Complex Proteins antibody
[Mab414]—ChIP
(7)
GraS signaling in Staphylococcus aureus is regulated by a single D35 residue in the extracellular loop
Microbiology Spectrum
October 17, 2023
Junho Cho, Adhar C. Manna, Helah S. Snelling, Ambrose L. Cheung
in TSBT at room temperature for 1 h and then incubated with a primary antibody [HA antibody (
Abcam
,
ab18181
) at
1:1,000
dilution to detect GraR, or GraS antibody (11) at
1:1,000
] in 5% BSA followed by
incubation
(8)
A fine-tuned yeast surface-display/secretion platform enables the rapid discovery of neutralizing antibodies against Clostridioides difficile toxins
Microbial Cell Factories
September 25, 2023
Ying Sun, Yongrong Zhang, Hua Yu, Ashley Saint Fleur, Di Yu, Zhiyong Yang, Hanping Feng
To evaluate surface display by
flow cytometry
, the cells were incubated with mouse anti-HA (
Abcam
,
ab18181
,
1:1000
) and
goat
anti-
human
KC (
Southern Biotech
,
2060-01
,
1:1000
) followed by secondary
labeling
with
(9)
Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function
Redox Biology
September 1, 2023
Fanglin Niu, Zhuozhuo Li, Yuanyuan Ren, Zi Li, Hua Guan, Yang Li, Yan Zhang, Yirong Li, Junle Yang, Lu Qian,
et al.
(
14225-1-AP
), and
p70 S6K
(
14485-1-AP
) were from
Proteintech
(Wuhan, China);
mouse
antibodies against HA (
ab18181
) and LAMP1 (ab253630) were from
Abcam
(Cambridge, UK);
mouse
antibody against β-actin (
TA811000
) was
(10)
Pharmacological inhibition of BAP1 recruits HERC2 to competitively dissociate BRCA1–BARD1, suppresses DNA repair and sensitizes CRC to radiotherapy
Acta Pharmaceutica Sinica B
August 1, 2023
Xin Yue, Tingyu Liu, Xuecen Wang, Weijian Wu, Gesi Wen, Yang Yi, Jiaxin Wu, Ziyang Wang, Weixiang Zhan, Ruirui Wu,
et al.
MA, USA); antibody to β-tubulin (No. T0023; WB
1:1000
) from Affinity Biologicals; antibody to HA (No.
ab18181
; WB
1:1000
; IP
1:1000
), RAD51 (No.
ab63801
; WB
1:1000
; IF
1:200
) from
Abcam
;
BAP1
(No.
sc-28283
; WB
(11)
The Piezo channel is central to the mechano-sensitive channel complex in the mammalian inner ear
Research Square
July 13, 2023
Jeong-Han Lee, Cristina Perez-Flores, Seojin Park, Hyo Jeong Kim, Yingying Chen, Mincheol Kang, Jennifer Kersigo, Jinsil Choi, Phung N. Thai, Ryan Woltz,
et al.
(EX-
Mm02159
-M56) and
mouse
Cib2 (CS-
Mm07673
-M02–01J). Antibodies were as follows: anti-HA (
Abcam
, cat no.
ab9110
,
ab18181
), anti-His (
Abcam
, cat no.
ab18184
,
ab9108
), anti-Myc (
Abcam
, cat no.
ab32
,
ab9106
), anti-mCherry (
Abcam
,
(12)
Human atlastin-3 is a constitutive ER membrane fusion catalyst
The Journal of cell biology
July 3, 2023
Bryce S, Stolzer M, Crosby D, Yang R, Durand D, Lee TH.
HA
transfections
, cells were fixed as described above and then stained with mouse anti-HA antibody (
ab18181
;
1:500
;
Abcam
) in blocking solution (PBS containing 0.2 M glycine, 2.5% calf serum, and 0.1% Triton
(13)
SKP2 Contributes to AKT Activation by Ubiquitination Degradation of PHLPP1, Impedes Autophagy, and Facilitates the Survival of Thyroid Carcinoma
Molecules and Cells
June 30, 2023
Yuan Shao, Wanli Ren, Hao Dai, Fangli Yang, Xiang Li, Shaoqiang Zhang, Junsong Liu, Xiaobao Yao, Qian Zhao, Xin Sun,
et al.
CST
),
mTOR
(#
2972
,
1:1,000
; CST), phosphorylated-
mTOR
(#
5536
,
1:1,000
;
CST
). Antibodies against HA tag (
ab18181
,
1:2,000
;
Abcam
), Myc tag (
ab9106
,
1:1,000
;
Abcam
), Flag tag (
ab125243
,
1:2,000
;
Abcam
).
Immunohistochemistry (IHC
(14)
The Effect of Outer Space and Other Environmental Cues on Bacterial Conjugation
Microbiology Spectrum
June 15, 2023
Bar Piscon, Eliana Pia Esposito, Boris Fichtman, Guy Samburski, Lihi Efremushkin, Shimon Amselem, Amnon Harel, Galia Rahav, Raffaele Zarrilli, Ohad Gal-Mor
Laboratories) as we previously described (66). Blots were probed with anti-HA tag antibody (
Abcam
;
ab18181
, diluted
1: 1,000
). Goat anti-
mouse
antibody conjugated to
horseradish
peroxidase (
Abcam
;
ab6789
, diluted
(15)
Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family
Nature communications
May 30, 2023
Deshmukh FK, Ben-Nissan G, Olshina MA, Füzesi-Levi MG, Polkinghorn C, Arkind G, Leushkin Y, Fainer I, Fleishman SJ, Tawfik D,
et al
.
used for
Western blots
include anti-HA(R) rabbit (
1:6000
,
ab9110
, Abcam), anti-HA(M)
mouse
(
1:1000
,
ab18181
,
Abcam
), anti-
PSMD1
(
1:1000
,
ab2941
,
Abcam
), anti-PSMD2 (
1:1000
,
PAB6715
,
Abnova
), anti-PSMA3 (
1:500
,
(16)
Insights into the expanding intestinal phenotypic spectrum of SOCS1 haploinsufficiency and therapeutic options
Journal of clinical immunology
May 9, 2023
Rodari MM, Cazals-Hatem D, Uzzan M, Martin Silva N, Khiat A, Ta MC, Lhermitte L, Touzart A, Hanein S, Rouillon C,
et al
.
for 1 h,
membranes
were incubated with
anti-SOCS1 (A156
, #
3950
Cell Signaling
), anti-HA tag (HA.C5,
ab18181
,
Abcam
), anti-
STAT1
(9H2, #
9176
,
Cell Signaling
), anti-pSTAT-1 (58D6, #
9167
,
Cell Signaling
), anti-
STAT5
(17)
Mutating novel interaction sites in NRP1 reduces SARS-CoV-2 spike protein internalization
iScience
April 21, 2023
Debjani Pal, Kuntal De, Timothy B. Yates, Jaydeep Kolape, Wellington Muchero
REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, HA.C5,
Abcam
,Cat #
ab18181
; RRID: AB_444303 Lamp1,Abcam,Cat #24170; RRID: AB_775978 Flag-M2,Sigma,Cat# SLBT7654;RRID: AB_259529 β-Actin,
Sigma
,Cat#
(18)
HY5 functions as a systemic signal by integrating BRC1-dependent hormone signaling in tomato bud outgrowth
Proceedings of the National Academy of Sciences
April 18, 2023
Han Dong, Jiachun Wang, Xuewei Song, Chaoyi Hu, Changan Zhu, Ting Sun, Zhiwen Zhou, Zhangjian Hu, Xiaojian Xia, Jie Zhou,
et al.
plants.
Transgenic plants
were identified
by western blot
using an anti-HA monoclonal antibody (
Abcam
,
ab18181
). Two independent homozygous lines of the F2 progeny of pBRC1–LOG4 and pBRC1–CKX7
transgenic plants
(19)
TCF7L2 acts as a molecular switch in midbrain to control mammal vocalization through its DNA binding domain but not transcription activation domain
Molecular Psychiatry
April 1, 2023
Huihui Qi, Li Luo, Caijing Lu, Runze Chen, Xianyao Zhou, Xiaohui Zhang, Yichang Jia
The antibodies: rabbit anti-
TCF7L2
(
Cell Signaling Technology
, #
2569
,
1:200
), mouse anti-HA (
Abcam
,
ab18181
,
1:500
), donkey anti-mouse IgG secondary antibody (Alexa Fluor 555,
Thermo Fisher Scientific
,
A31570
,
(20)
The plant unique ESCRT component FREE1 regulates autophagosome closure
Nature Communications
March 30, 2023
Yonglun Zeng, Baiying Li, Shuxian Huang, Hongbo Li, Wenhan Cao, Yixuan Chen, Guoyong Liu, Zhenping Li, Chao Yang, Lei Feng,
et al.
anti-ATG8 (
Agrisera
,
AS142769
), anti-GFP (Chromotek, 029762), anti-RFP (
Abcam
,
ab125244
), anti-HA (
Abcam
,
ab18181
), anti-MYC (
Santa Cruz
,
SC789
), and anti-SnRK1α1 (
Agrisera
,
AS10919
). In vivo phosphorylation
assay
In
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Anti-HA tag antibody [HA.C5] from Abcam
Supplier Page
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