Fig 2: Relationship between true and estimated percentage of cross-species transmission using VNTRs when the same strain is introduced. The simulated percentage of CST, ?, compared with its estimation, , using the MPR algorithm in a scenario where the strains introduced in each species were identical. Colored points represent each of the 200 simulations per value of ß, whereas each line illustrates the average relationship between the realized and estimated value (points averaged over the same value of ß). The straight line represents a theoretical un-biased estimation. In (A) 10 loci were used, with the average number of total mutations accumulated since introduction equal to 22, 214 and 2145. In (B) 50 loci were used, with the average number of total mutations accumulated equal to 102, 1013 and 10045. A zoomed plot of 0-10% CST is shown for (A) and B in (C) and (D), respectively.

Fig 3: Confirmation of two eEF2K targets identified via stable isotope labeling of amino acids in cell culture (SILAC)/bio‐orthogonal non‐canonical amino acid tagging (BONCAT). (a) Experimental design for labeling, isolating, and identifying newly synthesized proteins. (b) Representative western blots for N‐ethylmaleimide sensitive fusion protein (NSF) and PPP1CC. (c) Quantification of western blot data. *p < 0.01 via 2 × 2 (bicuculline × eEF2K inhibitor) anova; n = 8–9.