Fig 1: The roles of RAGE and AXL in promoting intravasation in GemOE tumor cells. (A) The number of Gem240 cells (Gem257 cells showed identical results) attached to non-coated or LMN I-, COL IV-, or FN-coated wells 24 h after platting followed by washing and counting of attached cells in at least 4 HPFs. Each assay was performed 3 separate times, each in triplicates. (B) The expression of the indicated proteins in the indicated cell lines following 24 h growth in Dox-containing SF-media followed by a switch to Dox-containing SF-media supplemented with none, or Gas6 plus vehicle, or plus R428 for 24 h. Note, the actin blot is the same as in Fig. 6F because it is from the same experiment. Percentage of AXL+/ß1-integrin+ populations (red), the AXL+/CD151+ populations (white), or ß1-integrin+/CD151+ populations (black) derived from FACS analysis of non-permeabilized Dox-induced Gem197 (C), Gem240 (D), and Gem257 (E) treated as indicated. Each assay was performed 3 separate times, each in triplicates. (F) Schematic representation of the data in the entire study as well as future directions.