Fig 2: p120 catenin negatively regulate the glucose stimulated insulin secretion. INS-1E cells were transfected with either control siRNA or three different siRNAs targeting Ctnnd1. After serum starvation, cells were treated with either 0.5 mM glucose or 10 mM glucose. A, cell lysates were subjected for Western blot analysis using p120 catenin and ß-actin antibodies and (B) Western blot images were quantified. C, secreted insulin concentrations in the supernatants of siRNA transfected cells were measured. D, total insulin content in the p120 catenin transfected cells were measured using total cell lysates. Results are mean ± SEM of at least three independent experiments *p < 0.05 and **p < 0.01 compared with control siRNA as assessed by two-way ANOVA with LSD post hoc analysis. #p < 0.05 compared with control siRNA as assessed by t test. LSD, least significance difference.