Fig 2: The expressions of BCR-ABL oncoprotein and its downstream signaling molecules were decreased in RFNs treated CML cells. K562 and K562/G01 cells were co-delivered with gRNA-17 plus donor, RFNs-half plus donor, RFNs plus donor, respectively. Proteins were extracted after 60 h of transfection and analyzed by western blot. Expressions of p-BCR-ABL and BCR-ABL were reduced in RFNs plus donor group (a). The activated phospho-CRKL, phospho-ERK and phospho-STAT5 were down-regulated in RFNs plus donor group (b)

Fig 3: Alterations in the cell junction and adhesion network. (A) An altered PPI network involved in cell junction and adhesion functions. Red-dotted rectangle indicates the interaction between CRKL and VEGR1/FLT1. (B) The centrality measure analysis of the contribution of each node to the PPI network. The x-axis is the “diffusion degree score” (see the method) and the y-axis is the nodes (gene symbols) ranked according to their diffusion degree score.